中国临床医学影像杂志2017，Vol.28Issue(7)：480-483,4.
99Tcm-MAG3-isoDGR-2C在荷瘤鼠体内分布和显像的研究
Study of the biodistribution and imaging of 99Tcm-MAG3-isoDGR-2C in tumor bearing mice
摘要
Abstract
Objective:To study the feasibility of using labeled MAG3-isoDGR-2C with 99Tcm as a potential molecular imaging agent for ανβ3 targeting.Method:The MAG3-isoDGR-2C was labeled with 99Tcm using glucoheptonate transformation and complexation.S180 tumor bearing mice were divided into 5 groups with 5 mice each.At 30 min,60 min,120 min,240 min and 360 min after the injection of 99Fcm-MAG3-isoDGR-2C,the mice were sacrificed by decapitating.The biodistribution in the organs,T/B and T/M was calculated.SPECT imaging was performed at different time points after injection in 5 tumor bearing mice,and the ratio of T/NT were calculated by using ROI.Results:The MAG3-isoDGR-2C was labeled with 99Tcm effectively and simply,and the radiation chemical purity could reach to 94.34％ without purification.The biodistribution showed the tracer was cleared rapidly from blood and was mainly excreted through kidney.The radioactive uptake in heart,lung,spleen,stomach and bone was low,and the uptake in tumor could be visualized.The maximum ratios of T/B and T/NT were 1.75 and 10.50 respectively in the time of 4 hours after injection of the tracers.The SPECT images demonstrated the increased activity in the region of tumor with the maximum T/NT of 3.24±1.71.Conclusion:99Tcm-MAG3-isoDGR-2C can be made easily and show certain targeting ability to the malignant process,which proves its potentiality to become a new molecular imaging agent.关键词
肿瘤,实验性/动物,实验/鼠科/体层摄影术,发射型计算机,单光子/99m锝甲氧基异丁基异腈Key words
Neoplasms, experimental/Animals, laboratory/Muridae/Tomography, emission-computed, single-photon/Technetium Tc 99m sestamibi分类
医药卫生引用本文复制引用
解朋,黄建敏,刘晓梅,魏玲格..99Tcm-MAG3-isoDGR-2C在荷瘤鼠体内分布和显像的研究[J].中国临床医学影像杂志,2017,28(7):480-483,4.基金项目
河北省自然科学基金(H2014206286). （H2014206286）
