中国免疫学杂志2017，Vol.33Issue(8)：1177-1180,4.DOI:10.3969/j.issn.1000-484X.2017.08.011
连翘苷拮抗环磷酰胺所致小鼠免疫抑制的实验研究
Experimental study on phillyrin resist mice immunosuppression induced by Cyclophosphamide
摘要
Abstract
Objective:To study the effect of phillyrin on immune function in mice treated with cyclophosphamide.Methods: Mice model of immunosuppression was induced by intraperitoneal injection of cyclophosphamide,the mice in the experimental group received phillyrin with 50,100 and 150 mg/kg doses by gavage.While model group and control group received normal salin,all treatments continued 7 d.The thymus and spleen organ coefficients was measured by conventional methods.The phagocytosis of peritoneal macrophages was measured by FITC microspheres.The levels of IL-2,IL-4 and cAMP in peripheral blood were detected by ELISA.The effects of different doses of phillyrin on the above indexes were analyzed in immunosuppressed mice.Results: A mice model of immunosuppression was established after 7 d,the results showed that the immune organ index,phagocytic capacity of macrophages and the level of immune factors in serum could be improved,and the content of cAMP in peripheral blood lymphocytes was decreased in different degree by phillyrin.Conclusion: Phillyrin has the function of immune regulation,which can resist the immunosuppression of cyclophosphamide.关键词
连翘苷/小鼠/免疫抑制Key words
Phillyrin/Mice/Immunosuppression分类
医药卫生引用本文复制引用
于晓东,王立辛..连翘苷拮抗环磷酰胺所致小鼠免疫抑制的实验研究[J].中国免疫学杂志,2017,33(8):1177-1180,4.基金项目
本文为辽宁省科技厅2014年自然科学基金项目(No.2014022048). （No.2014022048）
