中国人兽共患病学报2017，Vol.33Issue(7)：575-582,8.DOI:10.3969/j.issn.1002-2694.2017.07.001
基于RNA-seq分析Eha调控迟缓爱德华菌抵抗酸化的作用
Deep sequencing analysis on transcriptomes of Edwardsiella tarda regulated by Eha following acidification
摘要
Abstract
Our studies tried to demonstrate Eha (Et haemolysin activator) could regulate the resistance of the bacterium against acidification to survive in the macrophage and explain its underlying molecular mechanism.When the bacteria infected the macrophages at time intervals,intracellular survival rate in bafilomycin-treated macrophages was higher than that with untreated cells,and the rate of wild type ET 13 was higher than that of its eha mutant,respectively (P＜0.05).The survival rate of the wild type was higher than that of the mutant under acid treatment (P＜0.05).To determine the conditions that induced the highest eha expression,we constructed a pMP220-Peha LacZ plasmid and determined the lacZ expression under different conditions.After exposure of pH6.3 medium for 2 h time,we performed the whole transcriptomic profiles of the wild type and mutant by RNA-sequencing.We identified 147 differentially-expressed genes ([log2 ratio| ≥1),113 and 34 of which were significantly up-and down-regulated,respectively in the mutant,comparing with the wild type.These findings were validated by qRT-PCR.GO functional analysis revealed that these genes were divided into 25 categories,including the bacterial catalysis,cellular composition,combination,localization,metabolism,processing,and transportation.Based on the KEGG database,these genes were distributed in 55 pathways,such as two-component system,ABC transporters,and microbial metabolism in diverse environments.Overall,Eha is an important regulator to affect all kinds of target genes and pathways for E.tarda to adapt to an acid environment.These results could be helpful for further investigations of the mechanisms by which E.tarda survives in macrophages.关键词
eha基因/RNA-sequencing/E.tarda/酸化Key words
eha gene/RNA-sequencing/Edwardsiella tarda/acidification分类
医药卫生引用本文复制引用
刘念,李玉红,郑恩金,高大庆,陆承平..基于RNA-seq分析Eha调控迟缓爱德华菌抵抗酸化的作用[J].中国人兽共患病学报,2017,33(7):575-582,8.基金项目
Supported by the National Natural Science Foundation (No.31570124)国家自然科学基金(No.31570124)资助 （No.31570124）
