中国人兽共患病学报2017，Vol.33Issue(7)：583-587,5.DOI:10.3969/j.issn.1002-2694.2017.07.002
液氮保种对田鼠巴贝虫标准株存活力的影响
Influence of liquid nitrogen cryopreservation to survive capability of Babesia microti standard strain
摘要
Abstract
We discussed the influence of liquid nitrogen cryopreservation to survive capability of Babesia microti standard strain.The whole blood of mice infected with Babesia microti was put in liquid nitrogen to cryopreservation for 1 month,3 months,6 months,9 months,the whole blood was get out respectively and recovery at room temperature,and infected 3 mice respectively,100 μL/ mouse (the first generation after redissolution,the experiment group).In the same time,3 mice were also infected with Babesia microti as the animal conservation control group.When the infection rate was at a high level,the whole blood of the experiment group mice were injected into 3 normal BALB/c mice (the second generation after redissolution),to observe the changes of the Babesia microti form and proliferation situation,and also to observe the infection rate of the first and the second generation after redissolution in different conserving time.Compared with Babesia microti of animal subcultivation,the form of Babesia microti of liquid nitrogen cryopreservation changed a little.Small trophozoites,annular trophozoites,schizont and immature and mature merozoite and other form can also be seen.Compared with Babesia microti of animal subcultivation,the first time to see the worms and the time attaining to the high infection level were 1 to 2 days later,but for the second generation after redissolution,it is the same.There was no significant difference in different conserving time of 1,3,6,9 months.The influence of liquid nitrogen cryopreservation to survive capability and worm form of Babesia microti is a little,so liquid nitrogen cryopreservation can be a better way to conserving Babesia microti.关键词
田鼠巴贝虫/液氮保种/动物保种/存活力Key words
Babesia microti/liquid nitrogen cryopreservation/animal conservation/survive capability分类
医药卫生引用本文复制引用
蔡玉春,陈家旭,卢艳,艾琳,吴芬,陈韶红..液氮保种对田鼠巴贝虫标准株存活力的影响[J].中国人兽共患病学报,2017,33(7):583-587,5.基金项目
Supported by the Tropical Diseases Resource Center Project of National Science and Technology Basic Conditions Platform Program (No.2016) and the Youth Fund of Shanghai Municipal Commission of Health and Family Planning (No.20154Y0155)国家科技基础条件平台专项寄生虫病和热带病科技资源中心项目和上海市卫计委青年基金项目(No.20154Y0155)联合资助(No.2016) （No.2016）
