中国人兽共患病学报2017，Vol.33Issue(7)：588-591,4.DOI:10.3969/j.issn.1002-2694.2017.07.003
肺炎支原体CARDS毒素蛋白多表位拼接抗原的表达及鉴定
Expression and identification of recombinant chimeric CARDS of Mycoplasma pneumoniae
摘要
Abstract
We expressed multi-epitope chimeric protein of CARDS toxin protein of Mycoplasma pneumonia (Mp) in prokaryotic cells,and purified and investigated its immunoreactivity.A recombinant multi-epitope chimeric gene including ten critical epitopes was connected by linker and cloned into prokaryotic expression vector pET-28a(+),and transformed into E.coli BL21(DE3) cells for expression under induction of IPTG.The antigenicity of expressed recombinant protein was identified with 6 × His monoclonal antibody and human positive serum by Western blot.The recombinant expression vector pET-CARDS was constructed and the about 30 kDa recombinant chimeric protein expressed in BL21(DE3) successfully.Western blot analysis showed that it can react respectively with 6 × His monoclonal antibodies and human positive serum.This study showed that the chimeric CARDS protein has an obvious immunoreactivity and a potential to be a new antigen for the diagnosis of Mp infection.关键词
肺炎支原体/CARDS毒素蛋白/多表位拼接抗原/表达/鉴定Key words
Mycoplasma pneumonia/CARDS toxin/chimeric/expression/identification分类
医药卫生引用本文复制引用
刘宝山,赵芝娜,赵雨杰,王桂珍..肺炎支原体CARDS毒素蛋白多表位拼接抗原的表达及鉴定[J].中国人兽共患病学报,2017,33(7):588-591,4.基金项目
Supported by the Department of Science and Technology of Liaoning Province (No.20122225019)辽宁省社会科学发展基金(No.20122225019)项目资助 （No.20122225019）
