中国人兽共患病学报2017，Vol.33Issue(7)：607-612,6.DOI:10.3969/j.issn.1002-2694.2017.07.007
福建省鼠类感染巴尔通体调查及序列分析
Investigation and sequence analysis on Bartonella infection in rodents in Fujian Province, China
摘要
Abstract
We explored the status of Bartonella infection in rodents and the sequence characteristics of Bartonella in Fujian Province.Rodents in Fujian Province were captured by the night trapping method during 2014-2016.Information of the captured rodents on capturing dates and geographic locations,species,gender and ages were recorded.Heart blood samples were collected,from which the fragments of ghA gene and16S-23S rRNA gene of Bartonella were amplified by polymerase chain reaction (PCR).The PCR products were sequenced and the phylogenetic tree was constructed for homology analysis by biological analysis software.Data on infection rate were analyzed with Chi-square or Fisher exact test to indicate statistical significance.Results showed that 5 917 cages were laid and 381 rodents were captured,density of rodent was 6.44％.The overall Bartonella infection rate in rodents was 12.34 ％,while infection rate in domesticated rodents was 10.61％,with 11.30 ％ in Rattus norvebicus and 10.00％ in R.flavipectus.And the infection rate in wide rodents was 13.86％,with a rate of 22.86％ in Rattus losea and 18.00％ in R.fulvescens,respectively.The infection rate was higher in wild rodents than in domesticated rodents,however,no significant difference was found.The Western Fujian and Northern Fujian region had the higher infection rates of 20.00％ and 25.33％,and no infection was found in Southern Fujian region.The statistical analysis result revealed that a significant difference in infection rate among different region and habitats,but no significant difference in infection rate between male and female rodents,or among different ages.The BLAST results revealed the species to be B.tribocorum,B.elizabethae and B.grahamii.In conclusion,Bartonella infection is found in the rodents in Fujian Province and more attention should be paid on its impact on public health in the province.关键词
巴尔通体/鼠类/基因特征/福建Key words
Bartonella/rodent/genetic identification/Fujian分类
医药卫生引用本文复制引用
肖方震,林代华,周淑姮,徐国英,邓艳琴..福建省鼠类感染巴尔通体调查及序列分析[J].中国人兽共患病学报,2017,33(7):607-612,6.基金项目
Supported by the Medical Innovation Funding of Fujian Province (No.2012-CXB-12) and the Natural Science Foundation of Fujian (No.2017J01139)福建省医学创新课题(No.2012-CXB-12)和福建省自然科学基金项目(No.2017J01139)联合资助 （No.2012-CXB-12）
