中国人兽共患病学报2017，Vol.33Issue(7)：613-616,623,5.DOI:10.3969/j.issn.1002-2694.2017.07.008
一起急性腹泻疫情中检出的GI.8型诺如病毒的序列测定和分子特征分析
Complete genome sequence of a genogroup I geno type 8 norovirus identified in Huzhou, China
纪蕾 1吴晓芳 1陈莉萍 1韩建康1
作者信息
- 1. 湖州疾病预防控制中心,湖州 313000
- 折叠
摘要
Abstract
We identified and characterized the full-length genome of a GI.8 norovirus strain CHN/Huzhou/N10 isolated in an outbreak in Huzhou,China.The full-length genome of CHN/Huzhou/N10 was amplified using five pairs of primers which were designed according to the full-length GI norovirus genome sequences published in GenBank database.Multiple alignments were performed using DNAStar,the phylogenetic relationship of CHN/Huzhou/N10 and the representative NoV (Norovirus) strains from each genogroup were assessed using the software MEGA version 6.0.The viral genome of CHN/Huzhou/N10 was 7 740 nucleotides in length,which was consist of three ORFs spanning 5-5 404 nt (ORF1),5 388-7 019 nt(ORF2),and 7 019-7 660 nt (ORF3),respectively.Phylogenetic analysis based on polymerase and capsid sequences VP1 and VP2 region indicated that CHN/Huzhou/N10 belonged to GI.8 genotype.The amino acid sequence analysis of the VP1 region showed that CHN/Huzhou/N10 had 16 mutations compared with the representative strain Boxer/2001/US,12 of these variations were located in the P2 subregion.Moreover,a single amino acid change (T347S) occurred at histo-blood group antigen (HBGA) binding site Ⅱ and another single amino acid change (T397E) occurred at HBGA binding site Ⅲ.In this study,the first full genome of norovirus GI.8 isolated in Huzhou,China was extensively characterized.The data would be helpful not only for the epidemiology study,but also for the diagnostic tool development and effective vaccine design in the future.关键词
诺如病毒/GI.8/全基因组Key words
norovirus/GI.8/complete genome分类
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纪蕾,吴晓芳,陈莉萍,韩建康..一起急性腹泻疫情中检出的GI.8型诺如病毒的序列测定和分子特征分析[J].中国人兽共患病学报,2017,33(7):613-616,623,5.
