中国人兽共患病学报2017，Vol.33Issue(7)：617-623,7.DOI:10.3969/j.issn.1002-2694.2017.07.009
类泛素-蛋白酶体系统和药物外排泵抑制剂对结核分枝杆菌单纯利福平耐药性影响的研究
Effects of the Mycobacterium tuberculosis prokaryotic ubiquitin-like protein-proteasome system on the mono-resistant to rifampin resistance to Mycobacterium tuberculosis
摘要
Abstract
We studied the effect of the Mycobacterium tuberculosis prokaryotic ubiquitin-like protein-proteasome system on mono-resistant to rifampin resistance to M.tuberculosis.A resazurin-based assay was employed to evaluate minimum inhibitory concentration (MIC) and comparative research on mono-resistant to rifampin MTB with Pup,Dop,PafA,Mpa genes expression and deletion of the difference.Above testing strains,respectively,carbonyl cyanide chlorobenzene hydrazone (CCCP),reserpine (RP),verapamil (VP)and chlorpromazine (CPZ) were tested.We compared and analyzed the change of rifampicin MICs on the various strains.Compared with rifampin resistant MTB,overexpression of Pup,Dop,PafA and Mpa genes were able to make monorifampicin of M.tuberculosis to enhance resistance to rifampin.Deletion of Pup gene,Mpa gene,Dop gene,PafA gene significantly decreased the resistance to rifampicin alone MTB,and the P value was ＜0.05.Results indicated that 4 kinds of efflux pump inhibitors can reduce the degree of rifampin MIC in different strains.Through the factorial analysis,there were some interactions between MTB and PPS efflux pump inhibitors,and the P value was ＜0.05.MTB PPS has influence on mono-rifampin resistance to MTB and it may regulate the efflux pathway related protein to influence its resistance.关键词
结核分枝杆菌/类泛素-蛋白酶体系统/利福平/最小抑菌浓度/药物外排泵/耐药性Key words
Mycobacterium tuberculosis/prokaryotic ubiquitin-like protein-proteasome system/rifampin/minimum inhibitory concentration/active efflux/drug resistance分类
医药卫生引用本文复制引用
朱荟云,张万江,吴芳,吴江东,张杰,董江涛,章乐,张帅,张大龙,武青青..类泛素-蛋白酶体系统和药物外排泵抑制剂对结核分枝杆菌单纯利福平耐药性影响的研究[J].中国人兽共患病学报,2017,33(7):617-623,7.基金项目
Supported by the National Natural Science Foundation (Nos.81260261,81160192) and the High-level Personnel Scientific Research Projects of Shihezi University (No.RCZX201446)国家自然科学基金资助项目(No.81260261,No.81160192) （Nos.81260261,81160192）
石河子大学高层次人才科研启动项目(No.RCZX201446) （No.RCZX201446）
