中国兽医科学2017，Vol.47Issue(7)：817-824,8.DOI:10.16656/j.issn.1673-4696.2017.07.003
奇妙节丛孢的分离及对捻转血矛线虫幼虫和秀丽隐杆线虫捕食过程的观察
Isolation of Arthrobotrys thaumasia and observation of the predatory process of the fungal isolate to the larvae of Haemonchus contortus and free-living nematode Caenorhabditis elegans
摘要
Abstract
Nematophagous fungus Arthrobotrys thaumasia was isolated from the fresh feces,pasture soil,dung compost and barn soil,and the predating process of fungal isolates to nematodes was observed in this study.The study was divided into two steps:one was to isolate Arthrobotrys thaumasia using baited plate technique,and the other was to observe the dynamics predating process of A.thaumasia to the larvae of Haemonchus contortus and free-living nematode Caenorhabditis elegans through scanning electron microscopy (SEM).The results of fungal isolation indicated that eleven isolates of A.thaumasia were isolated from 1 532 samples associated with cattle and sheep,and the detection frequencies in total samples were 0.71％(11/1 532).The results of SEM showed that at 6 h after the nematodes tested were added,A.thaumasia isolate produced predatory structures and initially caught them.The cuticle of second stage larvae(L2) was penetrated by the fungus at 8 h post-captured,the L2 surface shrinked clearly at 30 h,which indicated that its inside organs were digesting,and L2 was completely destroyed at 78 h.The third stage infective larvae(L3) was penetrated by the fungus at 12 h post-caputred,the surface of body was shrinked at 42 hour and L3 was completely destroyed at 84 h.At 7 h post-captured,C.elegans was penetrated by the fungus and the cuticle clearly shrinked on cuticle,it seriously collapsed at 12 h and was completely digested at 18 h.Those results suggest that the fungus isolated in the present study belonged to A.thaumasia,which was able to prey three kinds of nematodes mentioned above.However,the predatory time of A.thaumasia varied with different types of nematodes.关键词
奇妙节丛孢/捻转血矛线虫/秀丽隐杆线虫/扫描电镜Key words
Arthrobotrys thaumasia/Haemonchus contortus/Caenorhabditis elegans/scanning electron microscopy分类
农业科技引用本文复制引用
胡黔林,张海燕,王海玉,刘洋,蔡葵蒸,曹欣,王波波,许强,王帆,方文秀,蔡彬,张超,刘艳秋,曾嘉庆..奇妙节丛孢的分离及对捻转血矛线虫幼虫和秀丽隐杆线虫捕食过程的观察[J].中国兽医科学,2017,47(7):817-824,8.基金项目
西北民族大学国家级大学生创新创业训练计划资助项目(201610742065) （201610742065）
国家自然科学基金项目(31660708) （31660708）
