中国兽医科学2017，Vol.47Issue(7)：825-830,6.DOI:10.16656/j.issn.1673-4696.2017.07.004
抗贝氏隐孢子虫卵黄抗体的制备与鉴定
Preparation and identification of yolk antibody against Cryptosporidium baileyi
摘要
Abstract
In order to lay a foundation for analyzing the effect of anti-Cryptosporidium baileyi antibodies,immunoglobulin of yolk(IgY)specifically against Cryptosporidium baileyi was prepared and identified.The purified oocysts of Cryptosporidium baileyi was prepared into immune antigens,and injected into 150-day-old laying hens 6 times under the wings and thigh by muscle multi-point injection,each time interval of immunization was between 15 and 20 d.In results,the IgYtiters reached 1∶25 600 on day 120 post-lst-immunization,and C.baileyi oocysts could be specifically recognized by IgY antibody using immunofluorescence.IgY antibody specificity against C baileyi,Cryptosporidium andersoni,Isospora suis and swine Toxoplasma gondii was comparatively measured by the indirect ELISA and indirect immunofluorescence assay.In result,the obtained IgY antibody could specifically recognize G.baileyi oocysts.Indirect ELISA method was used to determine the activity of different temperature and pH,and the results showed that the obtained antibodies had good thermal stability and acid resistance.Overall,the laying hens could produce IgY antibodies with high quality and specificity after immune by C.baileyi oocysts antigens,which lay a foundation for the further application of anti-Cryptosporidium specific IgY antibodies in diagnosis and treatment of cryptosporidiosis.关键词
贝氏隐孢子虫/特异性IgY抗体/制备/鉴定Key words
Cryptosporidium baileyi/specific IgY/preparation/identification分类
农业科技引用本文复制引用
崔朝辉,邵兆霞,菅复春,王荣军,张龙现..抗贝氏隐孢子虫卵黄抗体的制备与鉴定[J].中国兽医科学,2017,47(7):825-830,6.基金项目
河南省科技创新杰出人才计划项目(34200510012) （34200510012）
十二五国家科技重大专项(2012ZX10004220-011) （2012ZX10004220-011）
