中国兽医科学2017，Vol.47Issue(7)：877-883,7.DOI:10.16656/j.issn.1673-4696.2017.07.013
犬细小病毒广西流行株的分离鉴定及生物学特性分析
Isolation and identification of canine parvovirus isolated from Guangxi and analysis of it's biological characteristics
摘要
Abstract
In order to understand the biological characteristics of Guangxi local canine parvovirus (CPV) strain,in this study,feces were collected from dogs suspected of CPV infection in Guangxi province and were inoculated with FK81 cells to isolate CPV.Research was also done on the biological characteristics and pathogenicity of the isolate with hemagglutination test,resiitance analysis,homology analysis and challenge test.The results showed that the typical CPE was spotted and the HA blood coagulation rate was 1∶512 after the cells were passaged to the third generation.The virus TCID50 of the fifth generation virus was 1× 10-36/0.1 mL,and the strains was anti-heat,anti-acid and insensitive to chloroform and aether.Round and non-enveloped particles about 25 nm in diameter were observed by electron microscope,1 419 bp and 1 254 bp bands were detected by specific primers against CPV.The results suggested that a canine parvovirus strain was successfully isolated.This strain,according to VP2 gene homology analysis,shared from 98.9％ to 99.8％ homology with reference strains reported in neighboring countries and our country.Phylogenetic analysis showed that the genotype of this isolated strain was CPV-2a subtype,and had the closest relationship with Thailand VT148 reference strain.267th,305th,324th and 440th amino acid of this strain were detected to have mutated,completely different from other isolates of CPV-2a subtype.Animal regression test results also suggested that this isolated CPV strain belonged to virulent strain.To sum up,in this research,an epidemic strain of canine parvovirus in Guangxi was successfully isolated,and the genetic variation of its major capsid protein VP2 gene was analysed.The results will provide basic information for the better prevention and control of canine parvovirus in the future.关键词
犬细小病毒/分离/生物学特性Key words
canine parvovirus/isolation/biological characteristics分类
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刘金凤,廖文军,陈凤莲,白安斌,钟华,凌丹,吴健敏..犬细小病毒广西流行株的分离鉴定及生物学特性分析[J].中国兽医科学,2017,47(7):877-883,7.基金项目
国家重点研发计划重点专项(2016YFD0501000) （2016YFD0501000）
