中国兽医科学2017，Vol.47Issue(7)：890-896,7.DOI:10.16656/j.issn.1673-4696.2017.07.015
鸡源多杀性巴氏杆菌的分离鉴定及其毒力基因的检测
Isolation, identification and virulence genes detection of Pasteurella multocida from chicken
摘要
Abstract
The liver of dead chickens was inoculated on serum agar plates,and single colonies were subsequently selected in accordance with Pasteurella characteristics for purification,staining and microscopy.DNA extracted from pure cultures was used for PCR amplification of 16S rRNA,kmt1 and capA,7 housekeeping genes for multi-locus sequence typing(MLST),and 12 virulence genes.Finally,animal pat-hogenicity experiment was carried out.Two strains of Gram negative bacilli were isolated with bipolar staining.Sequences of 16S rRNA and kmtl genes shared homology higher than 99％withPasteurella multocida,capsule serotype was identified as type A,and genotype were ST129.Except for toxA,ptfAand nanH,9 virulence genes were detected,and animal pathogenicity experiment showed high pathogenicity for chicken.Identification of two P.multocida strains can provide references for genetic characteristics study of avian isolates in China.关键词
多杀性巴氏杆菌/分离/PCR鉴定/毒力基因Key words
Pasteurella multocida/isolation/PCR identification/virulence genes分类
农业科技引用本文复制引用
郭伟娜,路振香,刘利晓,龚争..鸡源多杀性巴氏杆菌的分离鉴定及其毒力基因的检测[J].中国兽医科学,2017,47(7):890-896,7.基金项目
安徽省教育厅自然科学研究重点项目(KJ2016A822) （KJ2016A822）
安徽科技学院自然科学研究项目(ZRC2016497) （ZRC2016497）
