中国兽医科学2017，Vol.47Issue(7)：909-914,6.DOI:10.16656/j.issn.1673-4696.2017.07.018
樱桃谷肉鸭源细小病毒QH-L01株的分离鉴定及VP1基因序列分析
Isolation, identification and analysis of VP1 gene of a Cherry Valley duck-origin parvovirus strain QH-L01
摘要
Abstract
In April,2016,samples were collected from duck with beak atrophy and dwarfism syndrome on a duck farm in Qionglai,Sichuan Province.A Cherry Valley duck-origin parvovirus was isolated and named QH-L01 strain.In order to further understand the molecular characteristics and virulence of this virus,ELD50 was determined,the VP1 gene was sequenced and compared with that of other parvovirus strains.The result of ELD50 was 10-6.54 ELD50/0.2 mL.Nucleotide and amino acid analysis showed that the structural VP1 gene was 2203 bp in size,encoding for a protein of 734 amino acids,and the QH-L01 isolate VP1 gene shared 92.8％ to 99.8％ identity and 77.4％-87.3％ with that of GPV and MDPV,respectively.The mino acids shared 95.2％-99.5％ and 85.4％-91.1％ identity with that of GPV and MDPV,respectively.The results of animal experiment showed that there were obvious symptoms of duck-origin parvovirus BADS in the experimental groups,and there were no changes in the control groups,indicating the successful virus isolation and identification.关键词
樱桃谷肉鸭/细小病毒/同源性/遗传进化分析Key words
Cherry Valley duck/parvovirus/homology/phylogenetic analysis分类
农业科技引用本文复制引用
陈兵,徐婧,黄超,刘文涛,阳丹丹,陈镜妃,马文静,陈正礼,罗启慧..樱桃谷肉鸭源细小病毒QH-L01株的分离鉴定及VP1基因序列分析[J].中国兽医科学,2017,47(7):909-914,6.基金项目
国家科技支撑计划课题(2014BAI03B01) （2014BAI03B01）
国家重大科学仪器设备开发专项(2013YQ49085906) （2013YQ49085906）
