中国听力语言康复科学杂志2017，Vol.15Issue(4)：285-290,6.DOI:10.3969/j.issn.1672-4933.2017.04.011
新生豚鼠急性高胆红素血症模型的听性脑干反应特征
The Characteristics of Auditory Brainstem Response of Neonatal Guinea Pig Animal Model with Acute Hyperbilirubinemia
摘要
Abstract
Objective To investigate the characteristics of auditory brainstem responses of the neonatal P3 guinea pig hyperbilirubinemia animal model and to study the acting sites of bilirubin on the hearing system.Methods Seventythree newborn guinea pigs at P3 were randomly divided into the control group of 10,a low dose bilirubin group of 40 and a high dose bilirubin group of 23,to which normal saline solution and 75μg/g or 100μg/g bilirubin was injected through intraperitoneal pathway respectively.The latency and inter-wave latency of ABR as well as the threshold were monitored before and 1h,4h,and 8h after administration,respectively.8 hours later after bilirubin injection,the blood was collected from the heart and the total serum bilirubin (TSB) was measured.Results There were no obvious abnormalities in the latency,inter-wave latency and thresholds of ABR before and after injection of normal saline solution.In the low dose group,the latency and inter-wave latency of ABR were prolonged in 14 guinea pigs,but the threshold was not significantly increased.In the high dose group,the latency and inter-wave latency of ABR were prolonged and the threshold was increased in 10 guinea pigs,the degree of latency and the inter-wave latency of ABR were higher than of the lower dose group.Conclusion Hyperbilirubinemia may damage both the peripheral and low brain stem auditory systems.The changes of ABR wave latency and inter-wave period were more sensitive than the ABR threshold and total serum bilirubin,and were more suitable for the early detection of high bilirubin-induced auditory system injury.关键词
高胆红素血症/听性脑干反应/潜伏期/波间期/血清总胆红素Key words
Hyperbilirubinemia/Auditory brainstem response/Latency/Inter-wave latency/Serum total bilirubin引用本文复制引用
赵振鹿,丛涛,杨驹,张超,袁硕龙,赵立东,乔月华..新生豚鼠急性高胆红素血症模型的听性脑干反应特征[J].中国听力语言康复科学杂志,2017,15(4):285-290,6.基金项目
国家自然科学基金面上项目(81271082) （81271082）
国家973计划重大科学研究计划干细胞项目子课题(2012CB967901,2012CB967903) （2012CB967901,2012CB967903）
青年基金(81400472) （81400472）
北京科技创新基地培育与发展专项(z151100001615050) （z151100001615050）
