中国药业2017，Vol.26Issue(15)：1-4,4.DOI:10.3969/j.issn.1006-4931.2017.15.001
披针骨牌蕨总黄酮对高糖诱导血管内皮细胞氧化损伤的保护作用
Protective Effect of Total Flavonoids from Lepiologrammitis Diversa on High Glucose-Induced Oxidative Damage in Vascular Endothelial Cells
摘要
Abstract
Objective To investigate the protective effect of total flavonoids from Lepiologrammitis Diversa on high glucose-induced ox-idative damage in vascular endothelial cells. Methods To establish oxidative damage models of human umbilical vein endothelial cells ( HUVEC ) induced by high glucose and to carry out drug test. The models were divided into the normal control group ( the mass con-centration of glucose was 5. 5 mmol/L ) , high glucose injury group ( the mass concentration of glucose was 40 mmol/L ) , extract group ( the mass concentration of total flavonoids from Lepiologrammitis Diversa were 100, 200, 400 mg/L ) and positive control group ( the mass concentration of vitamin C was 30 mg/L ) . The cell survival rate was measured by MTT assay, the changes of release and activity of LDH, MDA, SOD, NO and NOS in cells were determined by diagnostic reagent kit. Results In the extract group, the release amount of MDA was decreased significantly, the release amount and activity of LDH was decreased significantly, the activity of SOD was in-creased significantly, and the quantity and activity of NO and NOS were increased significantly ( P < 0. 05 ) , in a certain concentration range, and the total flavonoids from Lepiologrammitis Diversa showed dose dependent relationship in a certain mass concentration range. Conclusion Total flavonoids from Lepiologrammitis Diversa have protective effect on oxidative damage induced by high glucose in HUVEC. The preliminary mechanism may be related to the increase of antioxidase activity, the scavenging of free radicals and the in-crease of antioxidant capacity.关键词
披针骨牌蕨总黄酮/人脐静脉血管内皮细胞/高糖/氧化应激Key words
total flavonoids from Lepiologrammitis Diversa/human umbilical vein endothelial cells/high glucose/oxidative stress分类
医药卫生引用本文复制引用
张伟,罗优,李亚婷,胡晓峰,唐伟军,彭梁杰..披针骨牌蕨总黄酮对高糖诱导血管内皮细胞氧化损伤的保护作用[J].中国药业,2017,26(15):1-4,4.基金项目
湖南省卫生计生委科研计划项目[ C2016047 ] （）
湖南省教育厅科学研究项目[ 16C1495 ] （）
湘南学院校级科研课题立项资助项目[ 2015XB10 ] （）
湖南省大学生研究性学习和创新性实验计划项目[湘教通〔2014〕248号-489 ]. （）
