中国药业2017，Vol.26Issue(15)：13-16,4.DOI:10.3969/j.issn.1006-4931.2017.15.004
不同迁徙能力奇异变形杆菌的比较蛋白质组学研究
Comparative Proteomics of Proteus Mirabilis with Different Migratory Abilities
唐雨 1刘艳霞 1刘俊康 1邓渝1
作者信息
- 1. 中国人民解放军第三军医大学药学院生药学与天然药物化学教研室,重庆 400038
- 折叠
摘要
Abstract
Objective To detect differentially expressed proteins of two different migratory abilities of Proteus Mirabilis through compara-tive proteomics, to provide an important basis for further study on drug resistance of Proteus Mirabilis. Methods LB medium was used to culture the inner and outer ring Proteus Mirabilis with different migratory ability, and the difference of migration ability between the two strains was determined by the ratio of colony diameter. Comparative proteomic analysis was used to detect the differences in overall protein expression between the two species of bacteria and to focus on the interaction between distinct classes of ribose pro-teins. Results The results showed that diameter of the inner colonies was small and the diameter of outer colonies was large. The colony diameter ratio were 3. 84 ± 0. 56, compared with the inner ring, the outer ring of bacteria protein expression changes significantly, only the outer ring bacteria expressed 29 proteins, the outer ring of bacteria significantly down-regulation of 129 proteins and signifi-cantly up-regulation of 269 proteins. According to the analysis of ribosomal proteins, there were significant increase in expression of 34 kinds of proteins. Conclusion The morphology and protein expression of outer ring bacteria changed significantly, and the significant in-crease of ribosomal protein expression might be the cause of drug resistance.关键词
奇异变形杆菌/迁徙能力/比较蛋白质组学/耐药Key words
Proteus Mirabilis/migratory abilities/comparative proteomics/drug resistance分类
生物科学引用本文复制引用
唐雨,刘艳霞,刘俊康,邓渝..不同迁徙能力奇异变形杆菌的比较蛋白质组学研究[J].中国药业,2017,26(15):13-16,4.
