中国药业2017，Vol.26Issue(16)：1-5,5.DOI:10.3969/j.issn.1006-4931.2017.16.001
半夏泻心汤不同配伍组分对伊立替康致小鼠迟发性腹泻的影响
Clinical Evaluation of Different Combinations of Banxia Xiexin Decoction for Treating Delayed Diarrhea Caused by Irinotecan in Mice
摘要
Abstract
Objective To observe different combinations of Banxia Xiexin Decoction(BXD) for treating delayed diarrhea caused by irinotecan(CPT-11) in mice,and to investigate the mechanism of compatibility. Methods ICR mice were intraperitoneal injected with CPT-1140 mg/kg for 4 consecutive to establish models of delayed diarrhea. Totally 90 ICR female mice were randomly assigned to normal group,model group,positive drug group,BXD group,and 5 different compatibility groups,10 cases in each group. The diarrhea models were established as the same above. The normal group was injected with the same amount of normal saline instead of CPT-11, and the positive drug group,BXD group,and different compatibility groups were treated with loperamide hydrochloride every day respec-tively for 8 days starting 4 days before CPT-11 was injected. The diarrhea index of each group was calculated and their body weight was recorded. Mice were killed on the 8th day after experiments and intestinal tissues were removed for histological studies. Contents of serum IL-15 and β-glucuronidase of intestine were investigated. Results Delayed diarrhea after injected CPT-11 was observed on mice. Compared to model group,the diarrhea index of mice for BXD group,Kuhan group,Xinwen + Kuhan group,and Kuhan + Buyi group were obviously decreased ( P < 0. 01),the weight loss also had obvious improvements,and the damage degree of intestinal mu-cosa under light microscope was milder than that in the model group,the content of β-glucuronidase of intestine were significantly de-creased( P < 0. 01),the levels of serum IL-15 were increased. Conclusion BXD group,Kuhan group,Xinwen + Kuhan group and Kuhan + Buyi group can prevent the delayed diarrhea caused by CPT-11. The results reflected the characteristics of "symptoms caused by cold factors and symptoms caused by heat factors were recuperated at the same time" in Banxia Xiexin Decoction.关键词
伊立替康/迟发性腹泻/半夏泻心汤/配伍机制Key words
irinotecan/delayed diarrhea/Banxia Xiexin Decoction/mechanism of compatibility分类
医药卫生引用本文复制引用
史家文,关焕玉,何洋,王小明,覃文婷,雒晓梅,宿美凤,岳佳菁,石钺..半夏泻心汤不同配伍组分对伊立替康致小鼠迟发性腹泻的影响[J].中国药业,2017,26(16):1-5,5.基金项目
国家自然科学基金项目[81274054] （）
"重大新药创制"科技重大专项[2012ZX09301002-001-027]. （）
