摘要
Abstract
Objective To compare the differences of the volatile components of the Tianma Pill were extracted by direct pulverized into fine powder and steam distillation method. Methods The structures were analyzed and identified by headspace GC-MS. The GC-MS conditions were set as follows:HP-5MS quartz capillary column(30 m × 0. 25 mm,0. 25 μm),the initial temperature was 40 ℃ and maintained for 1 min,the temperature was heating to 195 ℃ at a rate of 3 ℃ /min and maintained for 2 min,then heating to 240 ℃at a rate of 5 ℃ /min and maintained for 6 min until completion of analysis. The carrier gas was helium,the column flow rate was 1. 1 mL/min,the split ratio was 10:1,the inlet temperature was 250 ℃,the EI ionization source was 70 eV,the ion source tempera-ture was 230 ℃,the scan range of m/ z 45-500. Results Totally 37 compounds were detected from Tianma Pill powder,among them,only 26 compounds were identified,which accounted for 83. 48% of total volatile composition. Totally 32 compounds were detected from Tianma Pill volatile oil,among them,only 21 compounds were identified,which accounted for 87. 25% of total volatile composition. Conclusion The type of constituents contained in the volatile oil extracted by direct pulverized into fine powder and steam distillation methods are totally different,and GC-MS can be used to identify the structures and relative content of volatile components,the results of this study can provide an experimental basis for development and utilization of Tianma Pill. The proposed method could provide useful information for the basic study on gas chromatography fingerprint and the quality evaluation of Tianma Pill.关键词
天麻丸/粉末/挥发油/挥发性成分/顶空进样/气相色谱-质谱联用法Key words
Tianma Pill/powder/volatile oil/volatile components/headspace/GC-MS分类
医药卫生
