中国肿瘤生物治疗杂志2017，Vol.24Issue(7)：700-707,8.DOI:10.3872/j.issn.1007-385x.2017.07.002
长链非编码RNA XLOC_005009对食管鳞状细胞癌生物学特性的影响
Effect of lncRNA XLOC_005009 on biological properties of esophageal cancer cell
摘要
Abstract
Objective:To explore effect of long non coding RNA (lonRNA) of XLOC_005009 gene on biological properties,proliferation,migration,invasion,cell cycle and apoptosis in vitro,of esophageal cancer cell.Methods:The surgically resected cancer and para-cancerous tissues of 25 patients with esophageal cancer who hospitalized in the Tumor Institute of the 4th Hospital of Hebei Medical University during 2015 to 2016 were collected.Expressions ofXLOC_005009 gene in human esophageal cancer Eca109 and Kyse 170 line cells,the esophageal cancer and paracancerous tissues were detected by RT-PCR.pcDNA3.1-XLOC_005009 over-expression plasmid was structured and transfected into the Eca109 and Kyse170 cells.MTS,colony forming,scratching,Transwell chamber and flow cytometry assays were used respectively to check proliferation,cloning efficiency,migration,invasion,cell cycle and apoptosis of the cells,before and after transfection of the over-expression plasmid.Results:Expression of XLOC_005009 mRNA in the esophageal cancer tissue was obviously lower than that in the para-cancerous normal tissue (0.06±0.06 vs 0.21 ±0.19,P＜0.05),and expressions of XLOC_005009 mRNA in the esophageal cancer cells were all lower than that in the control group.In the Eca109 and Kyse170 cells transfected with the over-expression plasmid,expressions of XLOC_005009 gene were higher than those in the control groups (Eca 109 cell:039±0.17 vs 0.02±0.00;Kyse 170 cell:0.35±0.08 vs 0.01 ±0.01,all P＜ 0.05).And comparing with the control group,proliferation abilities of the Eca109 and Kyse 170 cells with over-expression of XLOC_005009 gene were significantly weakened (P＜0.05),their cloning efficiency evidently reduced,transmembrane cell numbers of the cells remarkably decreased (Ecal09 cell:146.40±34.47 vs 193.00±26.33;Kyse170 cell:157.80±32.51 vs 269.00±29.89,all P＜0.05),migration efficiency of the Eca109 cell didn't markly change,but that of the Kyse 170 cell obviously reduced;S phase cell ratio of the cells increased;effecting on apoptosis of the cells was not obvious.Conclusion:Low-expression of XLOC_005009 might be closely related to occurrence and development of the esophageal cancer.Over-expression of XLOC_005009 could inhibit proliferation,invasion and migration in vitro of the esophageal cancer cells.关键词
食管鳞癌/XLOC_005009基因/长链非编码RNAKey words
esophageal squamous cell carcinoma/XLOC_005009 gene/long non coding RNA (lncRNA)分类
医药卫生引用本文复制引用
刘胜男,徐凤楼,卢帆,董稚明,邝钢,郭艳丽,沈素朋,梁佳,郭炜..长链非编码RNA XLOC_005009对食管鳞状细胞癌生物学特性的影响[J].中国肿瘤生物治疗杂志,2017,24(7):700-707,8.基金项目
国家自然科学基金资助项目(No.81472335),河北省自然科学基金资助项目(No.H2015206196).Project supported by the National Natural Science Foundation (No.81472335),and the Natural Science Foundation of Hebei Province (No.H2015206196) （No.81472335）
