中国肿瘤生物治疗杂志2017，Vol.24Issue(7)：716-720,5.DOI:10.3872/j.issn.1007-385x.2017.07.004
GRHL3和c-Myc在食管鳞状细胞癌组织中的表达及其临床意义
Expression of GRHL3 and c-Myc in esophageal squamous cell carcinoma tissue and its clinical significance
摘要
Abstract
Objective:To study expression situation of GRHL3 and c-Myc in the neoplastic and para-carcinoma tissues of the patients with esophageal squamous cell carcinoma (ESCC) and its clinical significance.Methods:Pathological confirmed neoplastic and para-carcinoma tissues of the 64 patients with ESCC who were hospitalized in Department of chest surgery,the Fourth Hospital of Hebei Medical University for tumor resection during April 2015 to July 2016 were collected.Real-time PCR and immunohistochemistry assays were used to detect expressions of mRNA and proteins of GRHL3 and c-Myc genes.Relationship of the expressions of mRNA and proteins of GRHL3 and c-Myc genes with clinical features of the patients with ESCC were analyzed.Results:Comparing with the paracarcinoma tissues,positive expression levels of GRHL3mRNA and GRHL3 protein in the ESCC tissue were obviously raised (mRNA:2.85±2.83 vs 2.06±2.02,protein:81.30％ vs 25.00％,all P＜0.01),positive expression levels of cMyc mRNA and cMyc protein in the ESCC tissue also significantly risen (mRNA:5.13±5.11 vs 2.03±2.00,protein:42.20％ vs 20.30％,all P＜0.01).In the ESCC tissue,expression of GRHL3mRNA and expression of cMyc mRNA were positively correlated obviously (P＜0.05),expression of GRHL3 protein and expression of cMyc protein were also positively correlated significantly (P＜0.01).Expressions of GRHL3 and cMyc proteins were related to tumor infiltration range,lymph node metastasis,clinical staging and differentiation degree of the patients with ESCC (all P＜0.05).Conclusion:Expression levels of GRHL3 and cMyc in the neoplastic tissues of the patients with ESCC were significantly raised,positive correlation was found between the both expressions,the both expressions were closely related to partial clinicopathological features of the patients with ESCC,which could be key factors that affecting pathological process of the ESCC.关键词
食管鳞状细胞癌/c-Myc基因/GRHL3基因Key words
esophageal squamous cell cancer/c-Myc gene/GRHL3 gene分类
医药卫生引用本文复制引用
韩晓楠,邓佳,安入征,张翔宇,刘丽华..GRHL3和c-Myc在食管鳞状细胞癌组织中的表达及其临床意义[J].中国肿瘤生物治疗杂志,2017,24(7):716-720,5.基金项目
国家自然科学基金青年科学基金资助项目(No.H2012206135),河北省杰出青年基金资助项目(No.H2014206320).Project supported by the Young Scientists Program of the National Natural Science Foundation of China (No.H2012206135),and the Natural Science Foundation of Hebei Province (No.H2014206320) （No.H2012206135）
