中国肿瘤生物治疗杂志2017，Vol.24Issue(7)：727-732,6.DOI:10.3872/j.issn.1007-385x.2017.07.006
长链非编码RNA FENDRR对宣威肺癌XWLC-05细胞增殖、迁移及侵袭的影响
Effect of lncRNA FENDRR on proliferation, migration and invasion of the Xuanwei lung carcinoma XWLC-05 cell
摘要
Abstract
Objective:To detect expressions of FENDRR long non coding RNA (lncRNA) in the lung adenocarcinoma XWLC-05 cell line from the Xuanwei area and in the non small cell lung cancer (NSCLC) A549 cell line,and to explore its effect on proliferation,migration and invasion of the cell lines.Methods:Expressions ofFENDRR gene in the XWLC-05 and the A549 cells were detected by Real-time quantitative fluorescence PCR.Over-expression plasmid pEX-3-FENDRR and lentiviral vector LV3-FENDRR were structured,then respectively transfected into theXWLC-05 and the A549 cells.After the transfection,expressions of FENDRR in the XWLC-05 and the A549 cells were tested by real-time quantitative fluorescence PCR.MTS assay was used to examine proliferation of the cells and Transwell assay used to test migration and invasion abilities of the cells.Results:Expression of FENDRR gene in the XWLC-05cell was obviously lower than that in the A549 cell.Level ofFENDRR mRNA in the XWLC-05cell transfected with pEX-3-FENDRR was enhanced and,which in the A549 cell transfected with LV3-FENDRR reduced.Comparing with the control group,over-expression of FENDRR could significantly inhibit proliferation of the XWLC-05cell ([1.23 ± 0.13] vs [1.46 ± 0.25],P＜0.05),and reduce its abilities of migration and invasion (P＜0.05).Interference of FENDRR expression could promote proliferation ([0.85 ± 0.02] vs [0.77 ± 0.08],P＜ 0.05),migration and invasion of the A549 cell.Conclusion:Down-regulation of lncRNA FENDRR expression could related with occurrence and development of the Xuanweilung adenocarcinoma,which could play a role of tumor suppression in the process of tumor malignization and inhibit proliferation and metastasis of the tumor cells.关键词
宣威肺腺癌/长链非编码RNA/FENDRR基因/增殖/迁移/侵袭Key words
Xuanwei lung adenocarcinoma/long non coding RNA(lncRNA)/FENDRR/proliferation/migration/invasion分类
医药卫生引用本文复制引用
陈冉,王玉明,段勇,张艳亮,宋贵波,吴春燕,孙岩,杜娜,肖成..长链非编码RNA FENDRR对宣威肺癌XWLC-05细胞增殖、迁移及侵袭的影响[J].中国肿瘤生物治疗杂志,2017,24(7):727-732,6.基金项目
国家自然科学基金资助项目(No.81460064,No.81460325),云南省科技厅昆医联合专项应用基础研究基金资助项目(No.2015FB047)和云南省领军人才计划资助项目(L-201202).Project supported by the National Natural Science Foundation of China (No.81460064,No.81460325),the Science and Technology Department of Yunnan Province-Kunming Medical University Joint Special Application Basic Research Foundation(No.2015FB047)and the Leading Talent Plan of the Health System in Yunnan Province (L-201202) （No.81460064,No.81460325）
