中国肿瘤生物治疗杂志2017，Vol.24Issue(7)：742-747,6.DOI:10.3872/j.issn.1007-385x.2017.07.008
miR-134-5p对宫颈癌细胞增殖和凋亡的影响及其分子机制
Effect of miR-134-5p on proliferation and apoptosis of cervical carcinoma cell and its molecular mechanism
摘要
Abstract
Objective:To observe effect of miR-134-5p transfection on proliferation and apoptosis of cervical carcinoma cell and to verify its possible molecular mechanism.Methods:Eight pairs of cervical cancer and para-cancerous tissuesfrom the patients with cervical cancer who hospitalized in Center of Oncology,Renmin Hospital,Hubei University of Medicine during May to August 2016 were collected.miR-134-5p mimics were transfected into cervical carcinoma Hela and SiHa cells by lipofectomin 2000.MTT and colony formation assays were used to detect proliferation of cells.Flow cytometry (FCM)assay was used to test cell cycles and apoptosis of cells.Expressions of miR-134-5p mRNA in cervical carcinoma tissue and cell,and expression of EGFR mRNA in cervical carcinoma cell were detected by qRT-PCR assay.Expressions of EGFR pathway-related proteins in cervical carcinoma cell were examed by Western blotting assay.ResuRs:Expression of miR-134-5p mRNA in cervical carcinoma tissue was significantly lower than that in para-carcinoma tissue (P＜0.01).Comparing with the Hela and SiHa cells that transfected with miR-NC,expressions of miR-134-5p mRNA in the Hela and SiHa cells that transfected with miR134-5p mimics were obviously increased,proliferation abilities of the cells significantly reduced (at the 5th day of the transfection,Hela ce11:1.06±0.13 vs 1.32±0.07;SiHa cell:1.12±0.10 vs 1.42±0.12,all P＜0.05),apoptosis rates of the cells obviously increased (Hela cell:[26.53± 13.48]％ vs [3.25±1.74]％;SiHa cell:[30.49± 12.04]％ vs [5.12±2.86]％,all P＜0.05),number of formed colony decreased,ratio of G0/G1 phase cells increased,ratio of the cells in S and G2/M phase decreased,apoptosis rate of the cells enhanced (all P＜0.05),expressions of EGFR mRNA and EGFR protein in the cells were remarkably down-regulated,among which EGFR mRNA in the the Hela cell down 58％ (P＜0.01) and in the the SiHa cell down 41％ (P＜0.05),expressions of downstream target protein for EGFR,p-AKT,p-ERK/2 and Cyclin D 1,as well as pEGFR proteins were evidently down-regulated.Conclusion:miR-134-5p could significantly inhibit proliferation of the cervical carcinoma cells and promote their apoptosis,of which possible molecular mechanism might be inhibit activation of EGFR pathway through inhibiting expression of EGFR gene.关键词
微小核糖核酸-134-5p/宫颈癌/增殖/凋亡/表皮生长因子受体Key words
micro ribonucleic acid-134-5p (miR-134-5p)/cervical carcinoma/proliferation/apoptosis/epidermal growth factor receptor (EGFR)分类
医药卫生引用本文复制引用
汤继英,蔡晓军,汪选斌,曹风军,佐志刚,张莉,熊柳鑫..miR-134-5p对宫颈癌细胞增殖和凋亡的影响及其分子机制[J].中国肿瘤生物治疗杂志,2017,24(7):742-747,6.基金项目
湖北省教育厅科学技术研究资助项目(No.B2016139),湖北省高校优秀中青年创新基金资助项目(No.T201510).Project supported by the Scientific Technology Research Foundation of Hubei Provincial Education Department (No.B2016139) and Innovative Foundation for Excellent Young and Middle-aged Talents in Universities of Hubei Province (No.T201510) （No.B2016139）
