中国肿瘤生物治疗杂志2017，Vol.24Issue(7)：756-761,6.DOI:10.3872/j.issn.1007-385x.2017.07.010
黏蛋白1基因转染DC对乳腺癌细胞MCF-7裸鼠移植瘤的免疫抑制作用
Immuno-inhibitory effect of DC transfected with MUC1 gene on xenograft tumor of the breast carcinoma MCF7 cell in nude mouse
摘要
Abstract
Objective:To explore inhibitory effect of dentritic cell (DC) transfected with mucin 1 (MUC1) gene on xenograft tumor of human breast carcinoma MCF7 cell in nude mouse.Methods:DC of healthy adults were induced-cultured in vitro.Plasmid with human pcDNA3.1-MUC1 gene were transfected into the DC by a lipofection assay.ELISA assay was used to detect secretion ability of cytokines IL-12 and TNF-α by the transfected DC.LDH release assay was used to exam killing activity of specific cytotoxic T lymphocytes (CTL) induced by the transfected DC to the breast carcinoma MCF7 cell.The xenograft tumors of human breast carcinoma MCF7 cell in nude mice were treated by the DC transfected with MUC 1 gene,the DC transfected with empty plasmid and normal saline in subcutaneous injection,and growth inhibition of the xenograft tumors in nude mice were observed.Results:Secretion abilities of IL-12 and TNF-α by the DC transfected with pcDNA3.1-MUC 1 gene were obviously more enhanced than those by the DC transfected with empty plasmid (IL-12:[202.52±29.61] vs [10.83±1.02] pg/ml;TNF-α:[349.07±79.42] vs [9.26±1.52] pg/ml,all P＜0.01).Killing activity of the specific CTL induced by the DC transfected with pcDNA3.1-MUC1 genewas more obvious than that by the CTL of the control group,and the killing rates at effector/targetor ratio of 10∶1,5∶1 and 2.5∶1 were 56.2％,38.9％ and 25.8％ respectively (all P＜0.01).Inhibitory effect of the DC transfected with MUC 1 gene on the xenograft tumors of breast carcinoma MCF7 cell in nude mice was significantly stronger than that of the DC transfected with empty plasmid (P＜0.05).Conclusion:The DC transfected with MUC1 gene might induce specific CTL,which might have stronger anti-tumor immuno effect on the breast carcinoma MCF7 cell.关键词
MUC1基因/树突状细胞/基因转染/乳腺癌/免疫效应Key words
MUC1 gene/dendritic cell(DC)/gene transfection/breast cancer/immune efficacy分类
医药卫生引用本文复制引用
尹良伟,马海英,张利,王贺双,刘宇,于环,祝艳华,伍建林..黏蛋白1基因转染DC对乳腺癌细胞MCF-7裸鼠移植瘤的免疫抑制作用[J].中国肿瘤生物治疗杂志,2017,24(7):756-761,6.基金项目
大连市卫计委科研课题资助项目(No.2012-0407L).Project supported by the Scientific Research Foundation for Projects of Dalian City's Health and Family Plaming Commission (No.2012-0407L) （No.2012-0407L）
