摘要
Abstract
Objective:To explore expression of long strand non-coding ribonucleic acid (lonRNA) AK093987 in colon carcinoma tissues and its clinical significance.Methods:Cancer and para-cancer tissues of the 65 patients with carcinoma of colon who hospitalized in the 1st People's Hospital of Neijiang,Sichuan,during January 2011 toDecember 2015,as well as normal colon tissues of the 15 patients with congenital dilatation of colon,and perforation of colon,colonal twist and incarcerated hernia caused by various reasons were collected.Expressions of lncRNA AK093987 in cancer and para-cancer tissues of the 65 patients with carcinoma of colon and the colon cancer cells were detected by RT-PCR assay.Proliferation of the colon cancer LoVo cells that were transfected with siRNA AK093987 to down-regulate expression of lncRNA AK093987 was tested by CCK8 assay.Cji-Square test and Kaplan-Meier assay were used respectively to analyze association of expression of lncRNA AK093987 with clinical features,survival and prognosis of the patients with carcinoma of colon.The factors affecting DFS and OS of the patients with carcinoma of colon were analyzed by Cox regression analysis.Results:Expression of lncRNA AK093987 in the cancer tissues of colon was obviously higher than those in the para-cancer tissue and the normal colon tissues (7.125±1.398 vs 1.058±0.070 and 1.092±0.049,all P＜0.01).Proliferation of the LoVo cells down-regulating expression of lncRNA AK093987 significantly reduced (P＜0.05).Expression of lncRNA AK093987 was remarkably related to clinical staging,metastasis of lymph node,distant metastasis,differentiation of tumor,serum CEA level and survival status of the patients with carcinoma of colon (all P＜0.05).DFS and OS medians of the patients with high expression of lncRNA AK093987 were significantly shortened than those in the patients with low expression of lncRNAAK093987 (DFS:[13.00±1.49] months vs [27.01±1.87] months;OS:[27.00±3.32] months vs [43.72±3.08] months,all P＜0.01).Expression of lncRNA AK093987,distant metastasis and clinical staging were the independent prognostic factors of the patients with colon cancer (P＜0.05).In addition,down-regulation of lncRNA AK093987 did evidently inhibit proliferation of the colon cancer LoVo cells.Conclusion:lncRNA AK093987 might involve in development of the colon cancer.Expression of lncRNA AK093987 could be obviously correlated to clinical staging,metastasis of lymph node,distant metastasis,differentiation of tumor,serum CEA level and survival status of the patients with colon cancer,which might be a potential molecular marker for diagnosis and prognosis assessment of the colon carcinoma.关键词
长链非编码核糖核酸AK093987/结肠癌/无进展生存时间/总生存时间Key words
long strand non-coding ribonucleic acid AK093987 (lncRNA AK093987)/colon cancer/progression free survival (PFS)/overal survival (OS)分类
医药卫生
