中国中西医结合外科杂志2017，Vol.23Issue(4)：376-381,6.DOI:10.3969/j.issn.1007-6948.2017.04.011
葫芦素E联合顺铂或紫杉醇治疗非小细胞肺癌的体外研究
Effect of Cucurbitacin E combined Cisplatin or Paclitaxel in Human Non-small Cell Lung Cancer Cells
摘要
Abstract
Objective To study the in vitro anti-proliferative effects of Cucurbitacin E (CuE) combined with cisplatin (DDP) or paclitaxel (PTX) in human non-small cell lung cancer cell lines A549 and H460. Methods MTT assay was performed to examine the growth inhibitory effects of CuE, DDP, PTX ,and CuE combined with DDP or PTX on A549 and H460 cells. And the effect of the combination therapy was evaluated by combination-index method of CHOU and TALALAY. Results Inhibitory effects were found when these 3 drugs were used alone or together. The median effect concentrations of these 3 drugs decreased to various extents when the drugs were used together. CuE was synergistic with DDP in A549 cells. In H460 cells, majority of effect range of CuE was synergistic with DDP. In A549 and H460 cells,low concentration of CuE was synergistic with low concentration of PTX, and middle-high concentration of CuE was antagonistic to middle-high concentration of PTX. Conclusion The effects of combination therapy are different in different human non-small cell lung cancer cell lines. Reasonable combination can greatly reduce the dosage of the two drugs, thus reducing the side effects.关键词
葫芦素E/顺铂/紫杉醇,非小细胞肺癌/细胞增殖Key words
Cucurbitacin E/cisplatin/paclitaxel/non-small cell lung cancer/cell proliferation分类
医药卫生引用本文复制引用
杨艳,李嵚,温艳惠,郭海梅..葫芦素E联合顺铂或紫杉醇治疗非小细胞肺癌的体外研究[J].中国中西医结合外科杂志,2017,23(4):376-381,6.基金项目
国家自然科学基金资助项目(81001028) （81001028）
