中医药学报2017，Vol.45Issue(3)：13-16,4.
莱菔硫烷对C57BL/6小鼠急性酒精性肝损伤的保护作用
Preventive Effects of Sulforaphane on Acute Alcoholic Hepatic Injury Model of C57BL/6 Mice
摘要
Abstract
Objective:To investigate the effects of sulforaphane (SFN) on abnormal function of liver induced by alcohol.Methods:Male C57BL/6 mice were randomly divided into 5 groups (n =12),which were the negative control group,alcohol model group,and SFN pretreatment group (10 mg/kg,20 mg/kg,40 mg/kg).Mice were daily gavaged SFN with different dose for ten days.At the end of last intake of SFN,mice in groups of the alcohol model and SFN pretreatment were gavaged alcohol(5 g/kg) every 12 hours for 3 times.The whole experiment was ended after 4 hours of last alcohol intake.HE staining was performed to check the pathological changes of liver.Blood and liver homogenates were collected to measure the expression levels of the relevant indicators by semi-automatic biochemistry analyzer.Results:The acute hepatic injury model was successfully estabhshed in C57BL/6 mice,as shown by indicators of pathology and serum.SFN,in high dose especially could lessen the pathological damage induced by alcohol.Also SFN significantly inversed the activities of AST,ALT and ALP induced by alcohol.SFN obviously activated the anti-oxidant enzymes including SOD and GSH-Px.Additionally,SFN pretreatment could inhibit the alcohol-induced MDA level,products of lipid peroxidation in dose-dependent way.Conclusion:SFN can protect the functions of liver damaged by alcohol.The main mechanisms may be associated with enhancing the antioxidant-enzymes,which are in charge of the metabolisms of alcohol.关键词
莱菔硫烷/肝损伤/酒精代谢/抗氧化Key words
Sulforaphane/Hepatic Injury/Liver damage/Alcohol metabolism/Antioxidant分类
医药卫生引用本文复制引用
李宝龙,单毓娟,刘旭,李松滨,刘永武,姜波..莱菔硫烷对C57BL/6小鼠急性酒精性肝损伤的保护作用[J].中医药学报,2017,45(3):13-16,4.基金项目
黑龙江省教育厅科学技术研究项目(12541763) （12541763）
国家自然科学基金面上项目(81573135) （81573135）
