中医药学报2017，Vol.45Issue(3)：41-44,4.
毛脉酸模愈伤组织诱导及生物活性成分分析
Analysis of Induction and Bioactive Constituents of Rumex Gmelini Callus
摘要
Abstract
Objective:To establish a culture method of callus induction for Rumex gmelini.Methods:The callus inductions of different explants were compared under different hormone conditions.The content difference of seven kinds of components was also compared in the callus tissue and seedlings by HPLC method.Results:The callus of cotyledons grew early,with large quantity,fast proliferation,bright color,and full vitality,which was beneficial to the subculture and further culture of suspension cells.The cotyledons were induced well in No.2 culture medium (MS + 6-BA 3.0 mg /L +2,4-D 0.1 mg/L),with high induction rate and fast growth speed.The callus is yellow-green and loose,which was good for further culture of cell suspension.The peak areas of most chemical components in the callus were larger.In terms of the seven bioactive components,polydatin and resveratrol in the callus were less than those in the seedling root.The contents of the other five components in the callus,in which a little amount of musizin was especially detected,were more than those in the seedling roots.Conclusion:The research suggests that the cotyledon may be the explants for callus induction,optimal medium is No.2 culture medium (MS + 6-BA 3 mg/1 +2,4-D 0.1 mg/l).The callus contained all the chemical elements in the seedling roots,of which the contents of five components were higher than those in the seedling roots,a little amount of musizin was also found.关键词
毛脉酸模/愈伤组织/诱导Key words
Rumex gmelini/Callus/Induction分类
医药卫生引用本文复制引用
徐明远,王谦博,崔红花,谢彦梅,岳赛男,吴柏艳,郭盛磊,丁常宏,王振月..毛脉酸模愈伤组织诱导及生物活性成分分析[J].中医药学报,2017,45(3):41-44,4.基金项目
国家自然科学基金项目(30970300) （30970300）
哈尔滨科技创新人才研究专项基金项目(2010rfxxs031) （2010rfxxs031）
