中医药学报2017，Vol.45Issue(3)：95-99,5.
大孔吸附树脂富集青龙衣总多酚的工艺研究
Technic-process Study of Enriching Total Polyphenols from Juglans Regia L.
摘要
Abstract
The total polyphenols from Juglans regia L.was enriched with macroporous resin.The optimal macroporous resin was selected after the static adsorption and desorption tests,with which to optimize the separation process of total polyphenols from J.regia.The parameters of concentration,pH value,bed volume and flow velocity from the sample liquid,as well as the parameters of concentration,bed volume,flow velocity and the ratio of diameter to height(D/H)from the eluant were observed with the optimal macroporous resin.The results showed that the AB-8 macroporous resin had good qualities of adsorption and desorption.The preferr ed condition for the procedure was as follows:the concentration of sample liquid was 0.070 g/mL,pH =4.0,the bed volume =1 BV,the flow rate of adsorption =3 BV/h,the volume of water to remove impurities was 3 BV;70％ ethanol was taken as the eluant,bed volume =2 BV,t he flow rate of desorption =2 BV/h and D/H =1∶ 8.Under this condition,the content of total polyphenols had been increased from 0.13％ to 52.09％.The AB-8 macroporous resin could be effectively applied to purify the total polyphenols from J.regia.关键词
大孔吸附树脂/青龙衣/总多酚Key words
Macroporous resin/Juglans regia L./Total polyphenols分类
医药卫生引用本文复制引用
陈晓怡,折改梅,王炎,赵怡程,白少娟,何婷,常艳丽,姜蕊,万欣蕾,邢丹..大孔吸附树脂富集青龙衣总多酚的工艺研究[J].中医药学报,2017,45(3):95-99,5.基金项目
北京中医药大学创新创业项目(201510026048) （201510026048）
北京中医药大学自主选题中青年教师资助项目(2013-JYB[J]ZZ-JS-137) （2013-JYB[J]ZZ-JS-137）
榆林现代特色农业新技术研究与集成示范(2015CXY-13) （2015CXY-13）
