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聚乙烯醇降解菌HK1菌株鉴定与降解特性

刘云柯 周云横 陈少林 卫亚红

安徽农业科学2017,Vol.45Issue(22):5-8,19,5.
安徽农业科学2017,Vol.45Issue(22):5-8,19,5.

聚乙烯醇降解菌HK1菌株鉴定与降解特性

Identification and Degradation Character of a Polyvinyl Alcohol Degradable Bacteria HK1

刘云柯 1周云横 2陈少林 1卫亚红2

作者信息

  • 1. 西北农林科技大学生命科学学院,陕西杨凌 712100
  • 2. 西北农林科技大学旱区生物质能研究中心,陕西杨凌 712100
  • 折叠

摘要

Abstract

[Objective] The aim was to identify a polyvinyl alcohol degradable bacteria HK1 and study its degradation character.[Method] We took a strain HK1 which could grow with polyvinyl alcohol as the only carbon source,identified it through its morphology and physiochemical characteristics,studied the resistance to several kinds of antibiotics which were commonly used in the laboratory,and studied its degradation character through shake flask test.[Result] According to its morphology,physiochemical characteristics and 16S rDNA sequence analysis,HK1 was identified as Bacillus amyloliquefaciens.It could degrade 36.26% of PVA (1 g/L) after culturing at 30 ℃,180 r/min in shaking flasks.The optimal conditions for PVA biodegradation by the obtained strain were as followed:30 ℃,1 g/L PVA,pH 8.0,and 2 g/L yeast.[Conclusion] The results can provide reference for further research of the strain.

关键词

聚乙烯醇/解淀粉芽孢杆菌/菌株鉴定/生物降解

Key words

Polyvinyl alcohol/Bacillus amyloliquefaciens/Identification/Biodegradation

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引用本文复制引用

刘云柯,周云横,陈少林,卫亚红..聚乙烯醇降解菌HK1菌株鉴定与降解特性[J].安徽农业科学,2017,45(22):5-8,19,5.

基金项目

杨凌示范区科技计划项目(2015NY-07) (2015NY-07)

安徽农业科学

0517-6611

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