波谱学杂志2017，Vol.34Issue(3)：257-265,9.DOI:10.11938/cjmr20162528
固体核磁共振中膜蛋白双交叉极化效率与动力学参数相关的定量分析
Efficiency of Double Cross Polarization in Magic-Angle Spinning Solid-State NMR Studies on Membrane Proteins
摘要
Abstract
The efficiency of multiple-dimensional heteronuclear correlation experiments in membrane protein studies is partially determined by the efficiency of double cross polarization (DCP). DCP is a basic block in the pulse sequences to probe the connections between the 15N and 13C nuclei. In this study, three membrane proteins were studied experimentally to determine how the dynamics properties of the proteins affect the DCP efficiency in solid-state heteronuclear correlation experiments. Under the same experimental conditions, the AQPZ protein showed the highest DCP efficiency (31%), while the EV712B protein had the lowest efficiency (14%) and the DAGK protein in between (23%). Longitudinal lattice relaxation time in the rotating frame (T1ρ) and dipole coupling constant (DHN) were also measured for these proteins. It was found that the DCP efficiency depended strongly on T1ρ, but only weakly on DHN. Based on the experimental data, a model to correlate the T1ρ of protein to the DCP efficiency was established, with which the DCP efficiency could be predicted by measuring the T1ρ of membrane proteins.关键词
固体核磁共振(NMR)/魔角旋转(MAS)/膜蛋白/双交叉极化(DCP)/旋转坐标下的自旋-晶格弛豫时间(T1ρ)Key words
solid-state NMR/magic-angle spinning (MAS)/membrane protein/double cross polarization (DCP)/T1ρ分类
数理科学引用本文复制引用
孙毅,陈艳可,李建平,赵永祥,杨俊..固体核磁共振中膜蛋白双交叉极化效率与动力学参数相关的定量分析[J].波谱学杂志,2017,34(3):257-265,9.基金项目
国家自然科学基金资助项目(10675162). （10675162）
