波谱学杂志2017，Vol.34Issue(3)：266-274,9.DOI:10.11938/cjmr20162527
离子对人血清白蛋白影响的1H NMR研究
Effects of Metal Ions on Human Serum Albumin Studied by Radiation Damping Water-Ligand Observed via Gradient Spectroscopy
摘要
Abstract
Human serum albumin (HSA) contains many metabolite binding sites. It has been widely studied for its functions in drug transportation. Nuclear magnetic resonance (NMR) is a frequently used tool to study HSA. However, due to its high molecular weight, the NMR spectra of HSA acquired with the conventional methods are often crowded, making spectral assignment and data interpretation difficult. In this study, a new method, which combined radiation damping water-ligand observed via gradient spectroscopy (RD-WaterLOGSY) with transverse relaxation weighted (T2W) techniques, was proposed to simplify the NMR spectra of HSA. With the T2W-RD-WaterLOGSY technique, the effect of pH on HSA was studied, as well as the interactions between HSA and Zn2+. The results showed that the relative changes in chemical shift could be used as a probe to analyze the pH changes in HSA solution and the interactions between HSA and Zn2+.关键词
核磁共振(NMR)/T2W-RD-WaterLOGSY/人血清白蛋白(HSA)/Zn2+/相互作用Key words
NMR/T2W-RD-WaterLOGSY/human serum albumin (HSA)/Zn2+/interaction分类
数理科学引用本文复制引用
陈瑶,孙鹏,刘买利,张许..离子对人血清白蛋白影响的1H NMR研究[J].波谱学杂志,2017,34(3):266-274,9.基金项目
国家自然科学基金资助项目(21120102038,81227902,20875098,21075132) （21120102038,81227902,20875098,21075132）
国家重点基础研究发展计划("973"计划)资助项目(2013CB910200). （"973"计划）
