长春中医药大学学报2017，Vol.33Issue(4)：523-526,4.DOI:10.13463/j.cnki.cczyy.2017.04.003
电针对APP/PS1双转基因小鼠学习记忆能力及LC3Ⅱ、P62表达水平的影响
Effect of electroacupunctureure intervention on learning and memory ability, LC3 Ⅱ and P62 Expression level of APP/PS1 transgenic mice
摘要
Abstract
Objective To investigate the effect of electroacupunctureure on learning and memory ability and its mechanism related to autophagic receptor P62 in APP/PS1 Transgenic mice. Methods The 7-month-old APP / PS1 transgenic mice were randomly divided into model group and EA treatment group and negative transgenic mice were selected into the nomal control group. EA was applied to"Baihui"and"Yongquan"for 15min,once every other day for 6 weeks.The learning-memory ability was detected by using Morris water maze testing, hippocampal LC3 Ⅱ and P62 were detected using Western blot(WB). Results Results of Morris water maze test showed that the escape latency of model group increased compared with the control group (P<0.01), WB results showed that compared with the control group, the model group LC3 Ⅱ expression enhanced, P62 expression decreased (P<0.05); compared with the model group, EA group LC3 Ⅱ expression was decreased, P62 expression increased (P<0.05). Conclusion EA intervention can improve learning and memory abilities in APP transgenic model mice, which may contribute to its effect in inhibiting neuronal autophagy.关键词
阿尔茨海默病/APP/PS1/LC3Ⅱ/P62Key words
Alzheimer's disease/APP/PS1/LC3 Ⅱ/P62分类
医药卫生引用本文复制引用
高杨,李丽娜,毛颖秋,周英奕,张学婷,张磊,薛卫国..电针对APP/PS1双转基因小鼠学习记忆能力及LC3Ⅱ、P62表达水平的影响[J].长春中医药大学学报,2017,33(4):523-526,4.基金项目
国家自然科学基金资助项目(81273826). （81273826）
