长春中医药大学学报2017，Vol.33Issue(4)：541-543,3.DOI:10.13463/j.cnki.cczyy.2017.04.009
不同提取方法对哈蟆油中雌二醇含量的影响
Effects of different extraction methods on estradiol content
摘要
Abstract
Objective The estradiol in Oviductus Ranae was extracted by three different methods and the corresponding estradiol content was determined to optimize the best method for extracting estradiol from Oviductus Ranae. Methods ODS-C18 chromatographic column (250 mm×4.6 mm, 5μm) was used, the mobile phase consisted of water-acetonitrile-methanol (63: 32: 5); the detection wavelength was 280 nm; the column temperature was 30 ℃ ; the flow rate was 1.0 mL/min. Results Estradiol content was 7.5μg / g in 90% ethanol extraction method, 1.25μg /g in supercritical fluid extraction method (without entrainer), estradiol in supercritical fluid extraction Was 0.83μg /g. Conclusion The method of extracting estradiol from Oviductus Ranae by 90% ethanol is the best.关键词
哈蟆油/雌二醇/超临界二氧化碳流体萃取Key words
Oviductus Ranae/estradiol/supercritical carbon dioxide fluid extraction分类
医药卫生引用本文复制引用
李兴月,张楠,翁丽丽,邱智东,张强..不同提取方法对哈蟆油中雌二醇含量的影响[J].长春中医药大学学报,2017,33(4):541-543,3.基金项目
吉林省中医药科技项目(20150309009YY). （20150309009YY）
