福建农业学报2017，Vol.32Issue(6)：634-638,5.DOI:10.19303/j.issn.1008-0384.2017.06.012
水稻稻曲病菌的分离鉴定及室内毒力测定
Isolation, Identification and Fungicide Toxicity of Ustilaginoidea virens
摘要
Abstract
Two methods for isolating HP-7 (Ustilaginoidea virens) and 7 fungicides for controlling the false smut disease on rice caused by the pathogen were compared.Fungal spores at different maturation stages were separated by means of shaking or flame burning.HP-7 was verified by its morphology, pathogenicity, and rDNA-ITS sequences.The sensitivity of HP-7 toward and toxicity on the fungi by the fungicides were determined based on the fungal mycelium growth under treatment.It was found that the successful rate in separating the fresh yellow chiamydospores by shaking was up to 98.33%, which was better than the flaming method.The rDNA-ITS sequences of HP-7 were submitted to NCBI for a BLAST analysis that showed a 99% homogeny with U.virens.Combining that result with the morphological observations, HP-7 was verified to be the pathogen that caused the rice false smut.Among the fungicides tested, tebuconazole and azoxystrobin exhibited the highest inhibition rates on HP-7, with EC50 at 0.109 1 and 0.158 5 mg·L-1, respectively.It appeared that an effective spore separation method was identified, and the positive identification and appropriate selection on fungicide for effective control of the false smut disease on rice crops were obtained.关键词
稻曲病/稻绿核菌/分离鉴定/室内毒力Key words
rice false smut/Ustilaginoidea virens/identification/laboratory toxicity test分类
农业科技引用本文复制引用
胡贤锋,李荣玉,王健,李明,李思博,黄永会,吴帅,赵琪君..水稻稻曲病菌的分离鉴定及室内毒力测定[J].福建农业学报,2017,32(6):634-638,5.基金项目
国家重点研发计划项目(2016YFD0200500) （2016YFD0200500）
国家公益性行业(农业)科研专项(201503107) （农业）
贵州省科技支撑计划项目(黔科合支撑[2016]2534号) （黔科合支撑[2016]2534号）
贵州大学引进人才科研项目[贵大人基合字(2015)07号] （2015）
大学生创新创业训练计划项目[贵大(省)创字2016(026)] （省）
