福建农业学报2017，Vol.32Issue(6)：645-651,7.DOI:10.19303/j.issn.1008-0384.2017.06.014
长裙竹荪抑菌物质乙醇提取工艺及其抑菌效果
Optimization of Ethanol Extraction of Antibacterial Substances from Dictyophora indusial Fisscher
摘要
Abstract
The ethanol extraction of antibacterial substances from Dictyophora indusial fisscher was optimized using the yield and efficacy of the antimicrobial extract as indicators.On basis of the single factor experimental result, a quadratic general rotary unitized design was applied to finalize the optimization for the process.A regression analysis on the data obtained indicated that the processing temperature exerted the greatest effected on the amount of the extract to be obtained, and it was followed by time and material/solvent ratio.The conditions rendering a maximized yield included a temperature of 72℃, a duration of 1.5 h, and a material/solvent ratio of 1/25.The resulting mushroom extract showed varying antimicrobial activities on Staphylococus aureus, Bacillus thuringiensi, Escherichia coli, Bacillus magaterium, Salmonella pullorum, Salmonella enteritidis, and Bacillus subrilis, with the strongest inhibition effect against S.enteritidis.The research results provide a reference for ntibaterial substances from D.indusial fisscher being developed national food preservative in the industrialization.关键词
长裙竹荪/抑菌物质/乙醇/提取Key words
Dictyophora indusial fisscher/antibacterial substances/ethanol/extraction分类
轻工纺织引用本文复制引用
陈晓宁,郑丽珠,魏奇,林程,陈炳智,肖淑霞,江玉姬..长裙竹荪抑菌物质乙醇提取工艺及其抑菌效果[J].福建农业学报,2017,32(6):645-651,7.基金项目
国家科技支撑计划项目(2013BAD16B03) （2013BAD16B03）
福建省现代农业食用菌产业技术体系项目(K83139295) （K83139295）
