硅酸盐通报2017，Vol.36Issue(7)：2203-2208,6.
以凹土颗粒稳定的乳液为模板制备复合微球固定化酶
Preparation of Composite Microspheres with Palygorskite Based Pickering Emulsion as a Template and Its Immobilization of Lipase
摘要
Abstract
Organic/inorganic composite microspheres were prepared by using palygorskite-based Pickering emulsion as a template.The composite microspheres were applied as the carrier for immobilizing lipase.When the lipase concentration was 0.020wt％,the immobilization temperature was 45 ℃ and pH value was of 7.4,the immobilized effect was optimized,and the enzyme activity reached the maximum.The thermal stability,storage stability showed good performance.The enzyme activity of the reuse of the immobilized lipase after 3 times was still close to that of the free lipase.关键词
凹土/Pickering乳液/固定化/脂肪酶Key words
palygorskite/Pickering emulsion/immobilization/lipase分类
化学化工引用本文复制引用
沈敏,李东,李文轩,张鹏颖,倪伶俐,金叶玲,吴洁,陈静,丁师杰..以凹土颗粒稳定的乳液为模板制备复合微球固定化酶[J].硅酸盐通报,2017,36(7):2203-2208,6.基金项目
国家自然科学基金(21174046,51174096) （21174046,51174096）
