医学分子生物学杂志2017，Vol.14Issue(4)：210-216,7.DOI:10.3870/j.issn.1672-8009.2017.04.005
PARP-1抑制剂对人肝癌细胞HepG2增殖和迁移的抑制作用
Effects of Different PARP-Inhibitors on the Proliferation and Migration of Human Hepatocellular Carcinoma Cell Line HepG
摘要
Abstract
Objective It has been confirmed that the inhibitors of poly ADP-ribose polymerase-1 (PARP-1) affect the proliferation,apoptosis and invasion of tumor cells.However,the impact of the inhibitors of PARP-1 on hepatoma cells remains unclear.The purpose of this study was to investigate the effect of three kinds of PARP-1 inhibitors on the proliferation,apoptosis and migration of hepatocellular carcinoma cells in vitro.Methods MTT assay was performed to detect the proliferation of HepG2 cells after treatment with PARP-1 inhibitors,AG014699,BSI-201,and AZD-2281.Thereafter,AG014699 and BSI-201,which had the most suppressive effect,were used to treat HepG2 cells.Western blot assay was used to detect the protein expression of asepase3,casepase8,Bax,Bcl-2,PTEN,tissue inhibitor of metalloproteinase 3 (TIMP3),and matrix metalloprotease 3 (MMP3).Transwell assay was performed to detect the effect of AG014699 and BSI-201 on the migration of HepG2 cells.Results AG014699,BSI-201 and AZD-2281 all could inhibit the proliferation of HepG2 cells in a time-and concentration-dependent manner.The protein expression levels of caspase3,caspase8,Bax,PTEN,TIMP3 increased and those of Bcl-2 and MMP3 decreased,with the increasing of the drug concentration,in HepG2 cells treated for 48 h,which showed a significant difference as compared with the control group (P<0.01).Conclusion All PARP-1 inhibitors AG014699,BSI-201,and AZD-2281 could significantly inhibit the proliferation of HepG2 cells,with AG014699 and BSI-201 having better sensitivity.AG014699 and BSI-201 could significantly induce the apoptosis and inhibit the migration of hepatoma cells,which may be related to the alteration of apoptosis-related signaling pathway and the expression of migration-related proteins.关键词
PARP-1抑制剂/肝癌/增殖/凋亡Key words
PARP-1 inhibitor/hepatoma carcinoma cell/proliferation/apoptosis/migration分类
医药卫生引用本文复制引用
毛小荣,杜森荣,杨忠霞,张立婷,彭雪彬,蒋妮..PARP-1抑制剂对人肝癌细胞HepG2增殖和迁移的抑制作用[J].医学分子生物学杂志,2017,14(4):210-216,7.基金项目
甘肃省技术研究与开发专项计划(No.1305TCYA023)This work was supported by a grant from the Special Project for Technology Research and Development nof Gansu Province(No.1305TCYA023) （No.1305TCYA023）
