广西植物2017，Vol.37Issue(8)：969-978,10.DOI:10.11931/guihaia.gxzw201703006
低温胁迫对"繁景"杜鹃生理特性及叶片超微结构的影响
Effects of low temperature stress on physiological characteristics and ultrastructure of "Fanjing" Rhododendron leaves
摘要
Abstract
As a new kind of hybrid offspring, "Fanjing" Rhododendron was studied for its tolerance to low temperature, and it was used as a green plant in Ningbo and surrounding cities.We used the excellent strains of "Fanjing" Rhododendron as the material with pot experiment, and the method of artificial cooling to study different low temperatures (0,-3,-6 and-9 ℃), physiological and biochemical effects of growth state and leaf ultrastructure.The results showed that at the temperature-3 ℃ and 0 ℃, the chlorophyll content decreased slowly and before treatment did not change significantly at the temperature-9 ℃ and-6 ℃, the chlorophyll content was significantly lower than that before treatment and the control group, different low temperature treatments, photosynthetic rate of leaves decreased, until the end of the trial when the photosynthetic rate was directly proportional to the temperature.Under-9 ℃ and-6 ℃ low tempe-rature stress, the relative conductivity and malondialdehyde in leaves (Malondialdehyde, MDA) content increased most rapidly, and catalase (Cata-lase, CAT), peroxidase (Peroxidase, POD) and superoxide dismutase (Superoxide Dismutase, SOD) activity decreased significantly;at-3 ℃ and 0 ℃ treatment, the content of MDA increased significantly, but soluble protein CAT, POD and SOD activity did not change significantly.Lower temperature had great effect on the ultrastructure of leaves of Rhododendron, when the temperature was at-3 ℃ and 0 ℃, the cell structure was normal;under-6 ℃ treatment, fuzzy starting structure of thylakoid, starch grains and osmiophilic particles become larger and more;under the-9 ℃ treatment, the cell membrane began to disintegrate and chloroplast membrane a serious lack of damage severity, hollowing out, some cells even become empty cells.Comprehensive indicators of changes in the si-tuation, excellent strains of Rhododendron can tolerate a lower temperature of-6 ℃.Therefore, it can be used as a good candidate material for Rhododendron in Ningbo and surrounding cities.关键词
低温胁迫/杂交后代/生理特性/超微结构Key words
low temperature stress/hybrid progeny/physiological characteristics/ultrastructure分类
农业科技引用本文复制引用
明萌,何静雯,卢丹,谢晓鸿,吴月燕..低温胁迫对"繁景"杜鹃生理特性及叶片超微结构的影响[J].广西植物,2017,37(8):969-978,10.基金项目
宁波市重大科技专项项目 (2014C11002) （2014C11002）
浙江省重中之重学科"生物工程"开放基金 (KF2015008) [Supported by Ningbo Major Science and Technology Program (2014C11002) （KF2015008）
Top Priority of the Discipline "bioengineering" Open Fund of Zhejiang Province (KF2015008)]. （KF2015008）
