广西植物2017，Vol.37Issue(8)：1035-1042,8.DOI:10.11931/guihaia.gxzw201611001
培养条件对三七愈伤组织生长和皂苷积累的影响
Effects of culture conditions on the growth and accumulation of saponin in Panax notoginseng callus
摘要
Abstract
It is well known that the growth and secondary metabolites accumulation of plant cells cultured in vitro are determined by many factors, such as culture conditions, nutrients supply, phytohormone application, etc.This study aims to explore the affecting factors during Panax notoginseng callus culture.On the basis of MS, modulations of phytohormone combination, nitrogen resource and illumination strength were carried out, and then the changes in saponins content during culture of P.notoginseng callus were measured and analyzed with spectrophotometry and HPLC.The results showed that the culture conditions had an considerable effect on the saponin accumulation in Panax notoginseng callus, in detail, combination of phytohormone had significant effects on saponin content with a peak of 4.72% ± 0.29% from the callus cultured on MS supplemented with 0.5 mg·L-1 2,4-D+1.0 mg·L-1 6-BA;The total N source concentration was maintained at 60 mmol·L-1, the saponin accumulated at the highest level of 4.71% ± 0.17% in the callus under the ratio of NO3-/NH4+=7∶1 (45 mmol·L-1 KNO3+7.5 mmol·L-1 NH4NO3);When cultures grown in the light (1 000 lx and 500 lx for 12 h·d-1, respectively) versus dark, their saponin content were calculated as 1.94% ± 0.31%, 2.38% ± 0.12% and 3.57% ± 0.27%, respectively.In addition, greening and cell differentiation were observed on the surface of callus under light, might be the main cause resulting in the inhibition of saponins synthesis and accumulation in callus.Saponins of P.notoginseng have been acknowledged to be the major compounds functioning in medical treatment of diseases, herein they are employed as the indicator of callus evaluation.Four kinds of saponins including Rg1, Re, Rb1 and Rd were identified from both the P.notoginseng callus and root by HPLC, and R1 could not be detected in the former, however, presence in the latter, the reason for which needs to be further investigated.The above results are likely to provide technical support for the production of natural compounds through callus culture in large scale instead of field cultivation partly in the future, furthermore, which would also be a potential alternative to wild medicinal plant resources for its merits of sustainable and rational exploitation.关键词
激素配比/三七/愈伤组织/皂苷/组织培养Key words
ratio of phytohormones/Panax notoginseng/callus/saponins/tissue culture分类
农业科技引用本文复制引用
齐琳琳,李刚,刘志伟,金朝霞,张宗申..培养条件对三七愈伤组织生长和皂苷积累的影响[J].广西植物,2017,37(8):1035-1042,8.基金项目
河南省科技创新杰出人才基金(134200510002) [Supported by the Excellent Talents Fund of Henan(134200510002)]. （134200510002）
