广西植物2017，Vol.37Issue(8)：1060-1067,8.DOI:10.11931/guihaia.gxzw201609015
高良姜等五味山姜属中药乙酸乙酯部位的HPLC指纹图谱
HPLC fingerprints of ethyl acetate parts of five traditional Chinese medicines
摘要
Abstract
We established the HPLC fingerprints of ethyl acetate parts from five traditional Chinese medicines (Alpinia officinarum, A.galanga , A.katsumadai, A.galangal and A.oxyphylla) to study the similarities and differences with the original chemical compositions of Chinese medicines, and to explore the correlation between the genetic relationship and chemical compositions.We firstly analyzed by 95% ethanol extractive, and then explored the correlation between the genetic and chemical compositions using their extractive.The analyzed condition was as follows: C18 column (250 mm × 4.6 mm, 5 μm) by gradient elution with acetonitrile, 0.1% phosphoric acid solution as mobile phase at a flow rate of 1.0 mL·min-2, the detection wavelength at 260 nm and column temperature at 30 ℃.The data were evaluated using the "similarity evaluation system for chromatographic fingerprint of TCM" software.The similarities of ethyl acetate parts chemical compositions were as follows: 0.955, 0.805, 0.371, 0.794 and 0.345.The study showed a certain similarity between the chemical compositions in ethyl acetate parts with fingerprints characteristic peak.And the results may indicated high similarity among A.officinarum, A.galangal and A.katsumadai, while low similarity between Alpinia galanga and A.oxyphylla in fingerprints.The established HPLC fingerprint chromatogram of five traditional Chinese medicines was precise, reproducible and stable.In this study, the resulted also identified the nine common peaks, six strong peaks.The spectrum showed that A.officinarum and A.galangal share five common peaks;A.officinarum and A.galangal share three common peaks;A.galangal and A.galanga share two common peaks;A.officinarum and A.oxyphylla share three common peaks.The results showed that the method was stable, time-saving and reliable.The original chemical compositions have a certain correlation between traditional Chinese medicines (TCM).This study provides the information for exploring the relevance on genetic relationship of TCM and chemical compositions in Alpinia.关键词
山姜属/中药/乙酸乙酯部位/HPLC/指纹图谱Key words
Alpinia Roxb/traditional Chinese medicine/ethyl acetate parts/HPLC/fingerprint/determination分类
农业科技引用本文复制引用
秦华珍,李明芳,谭喜梅,翁铭钻,黄燕琼,谢鹏,龙小琴,罗君..高良姜等五味山姜属中药乙酸乙酯部位的HPLC指纹图谱[J].广西植物,2017,37(8):1060-1067,8.基金项目
国家自然科学基金(81360646) （81360646）
广西教育厅高校科研项目(LX2014163)[Supported by the National Natural Science Foundation of China(81360646) （LX2014163）
Scientific Research Program of Guangxi Education Department(LX2014163)]. （LX2014163）
