工业微生物2017，Vol.47Issue(4)：1-10,10.DOI:10.3969/j.issn.1001-6678.2017.04.001
甜叶悬钩子(Rubus suavissimus S.Lee)中UDP-糖基转移酶基因的克隆、表达及序列分析
Cloning, expression and sequence analysis of UDP-glycosyltransferases gene from Rubus suavissimus S.Lee
摘要
Abstract
Glycosylation is an important modification reaction in the biosynthesis of plant secondary metabolites.UDP-glycosyltransferases (UGT) consuming diterpenoid as substrates were scarcely reported.Based on transcriptic data of Rubus suavissimus S.Lee leaves,UGTs genes with potential diterpene catalytic activity was selected by bioinformatic analyses.Finally,18 UGT genes were successfully cloned and expressed in Escherichia coli.Preliminary sequence analysis of the cloned genes was performed.This study laid a foundation for further research and verification of the function of UGTs in Rubus suavissimus S.Lee.关键词
UDP-糖基转移酶/甜叶悬钩子/生物信息分析/克隆/异源表达Key words
UDP-glycosyltransferases/Rubus suavissimus S.Lee/bioinformatics analysis/cloning/heterogeneous expression引用本文复制引用
李微微,孙雨伟,杨靖亚,王勇..甜叶悬钩子(Rubus suavissimus S.Lee)中UDP-糖基转移酶基因的克隆、表达及序列分析[J].工业微生物,2017,47(4):1-10,10.基金项目
国家自然科学基金(31670099) （31670099）
上海市自然科学基金(14JC1406900 （）
17ZR1435000):中国科学院分子植物科学卓越创新中心部署项目(CEMPS2016004) （CEMPS2016004）
上海市科委工程中心能力提升项目(16DZ2280300). （16DZ2280300）
