哈尔滨商业大学学报（自然科学版）2017，Vol.33Issue(4)：406-410,5.
高效液相色谱法测定益肾蠲痹丸中的马兜铃酸A
HPLC determination of aristolochic acid in Yishen Juanbi pill
王崇益 1隽海龙 1李浩2
作者信息
- 1. 江苏正大清江制药有限公司,江苏 淮安223005
- 2. 淮安市第一人民医院,江苏 淮安223001
- 折叠
摘要
Abstract
To establish a sensitive and good reproducibility mehiod of determinating aristolochic acid A method for aristolochic acid A in Yishen Juanbi pill.RP-HPLC method was adopted.Diamonsil C18 column, 5 μm, 4.6×250 mm was used.The mobile phase was methanol-water-acetic acid (69∶30∶1) at a flow rate of 1.0 mL/min, and the column temperature was 35 ℃.The UV-detection wavelength was 250 nm.The results suggested that under the range from 0.011 to 0.304 μg and the regression equation Y =5 308 818Χ-4 676, the recoveries (n=9) of the method was 99.31%.The method was accurate, sensitive, specific, and suitable.关键词
马兜铃酸A/益肾蠲痹丸/含量测定/高效液相色谱法Key words
aristolochic acid A/Yishen Juanbi pill/content determination/HPLC分类
医药卫生引用本文复制引用
王崇益,隽海龙,李浩..高效液相色谱法测定益肾蠲痹丸中的马兜铃酸A[J].哈尔滨商业大学学报（自然科学版）,2017,33(4):406-410,5.
