国际眼科杂志2017，Vol.17Issue(9)：1639-1642,4.DOI:10.3980/j.issn.1672-5123.2017.9.08
氯化锂对人眼Tenon囊成纤维细胞TGF-β及CTGF的影响
Effects of lithium chloride on transforming growth factor beta and connective tissue growth factor in cultured human Tenon's capsule fibroblasts
摘要
Abstract
AIM:To research the effects of lithium chloride on transforming growth factor beta (TGF-β) and connective tissue growth factor (CTGF) in cultured human Tenon capsule fibroblasts (HTFs) and explore its mechanism.METHODS:HTFs were cultured and identified by vimentin staining with immunofluorescence and the morphological characteristics.The experimental group was processed 48h with LiCl in concentration of 80mmol/L, the control group without LiCl.The mRNA expression of TGF-β and CTGF in two groups were analyzed with real-time fluorescent quantitative polymerase chain reaction (real time-qPCR) and the protein expression was detected with Western blot.RESULTS:The cultured HTFs expressed TGF-β and CTGF.The mRNA expression of TGF-β and CTGF significantly decreased compared with the control group(t=20.042, 14.995, P<0.05).the protein expression of TGF-β and CTGF also decreased significantly compared with the control group(t=46.058、12.452, P<0.05)CONCLUSION:The cultured HTFs can express TGF-β and CTGF in mRNA and proteins' level.LiCl can reduce the expression of TGF-β and CTGF both in gene and proteins' level.LiCl has the potential to modulate wound healing for glaucoma filtration surgery.关键词
转化生长因子β/结缔组织生长因子/Tenon囊/成纤维细胞/氯化锂Key words
transforming growth factor beta/connective tissue growth factor/Tenon's capsule/fibroblasts/lithium chloride引用本文复制引用
卢素素,刘姗姗,范晓军,孙晓祥,卞江华,王继兵..氯化锂对人眼Tenon囊成纤维细胞TGF-β及CTGF的影响[J].国际眼科杂志,2017,17(9):1639-1642,4.基金项目
潍坊市科学技术发展计划项目 (No.2015WS017).Science & Technology Development Project of Weifang City, China (No.2015WS017) （No.2015WS017）
