农业环境科学学报2017，Vol.36Issue(8)：1672-1679,8.DOI:10.11654/jaes.2017-0249
QuEChERS方法结合高效液相色谱-串联质谱法同时测定土壤中20种抗生素
Simultaneous extraction and determination of antibiotics in soils using a QuEChERS-based method and liquid chromatography-tandem mass spectrometry
摘要
Abstract
The aim of this work was to develop a method, based on QuEChERS(quick, easy, cheap, effective, rugged, and safe), to extract and analyze residues of 20 antibiotics in soil. The developed method included extraction with an ACN:EDTA:McIlvaine buffer, clean-up with a dispersive solid phase extraction(d-SPE)adsorbent using primary secondary amine(PSA)and octadecylsilane(C18), and finally analyte determination using liquid chromatography- tandem mass spectrometry(LC- MS/MS). This work optimized different extraction methods and ratios of clean-up adsorbents to perform at seven spiking levels(2.0, 5.0, 10.0, 20.0, 50.0, 100.0, and 200.0μg·kg-1)with recoveries ranging from 61.4% to 118.9% with relative standard deviations below 20%(n=5). The limits of quantification(LOQ)of the methods were in the range of 2.0~5.0 μg·kg-1 for most analytes. Linear regression coefficients of greater than 0.990 were obtained. This method was applied to the analysis of real agricultural soil samples, confirming the feasibility of the method.关键词
QuEChERS/抗生素/多残留分析/土壤/液相色谱-串联质谱Key words
QuEChERS/antibiotics/multi-residue analysis/soil/LC-MS/MS分类
资源环境引用本文复制引用
孟明辉,贺泽英,徐亚平,王璐,彭祎,刘潇威..QuEChERS方法结合高效液相色谱-串联质谱法同时测定土壤中20种抗生素[J].农业环境科学学报,2017,36(8):1672-1679,8.基金项目
中国农业科学院创新工程项目Project supported:The Innovation Project of Chinese Academy of Agricultural Sciences （）
