水产学报2017，Vol.41Issue(8)：1192-1202,11.DOI:10.11964/jfc.20160310305
亚硝酸盐氮胁迫对鳙血液生化指标以及组织HSP70 mRNA表达水平的影响
Effects of nitrite nitrogen stress on blood biochemical indictors and HSP70 mRNA expression level of Aristichthys nobilis
摘要
Abstract
In order to study the effects of nitrite nitrogen stress on blood biochemical indictors and HSP70 mRNA expression level of Aristichthys nobilis [initial average weight (180.05±0.092) g], 170 individuals of A. nobilis were exposed to water body with nitrite nitrogen concentration of 48.634 mg/L for 96 hours, and then a 96-hour recovery experiment was carried out. Blood and tissues of gill, head kidney, intestines and spleen were sampled at 0, 6, 12, 24, 48, 72, 96 h after stress, and at 12, 24, 48, 72, 96 h after recovery. For the stress and recovery experiment process, the results showed serum cortisol and ALT activity significantly increased first, after being stressed for 6 h. Thereafter, serum GLU presented significant increase at 12 h. Whereafter, serum CHOL, TP and TG decreased significantly and T3, AST increased significantly, while serum LDL and HDL decreased significantly at 72 h. For the recovery experiment, A. nobilis were recovered from nitrite nitrogen stress after 96 h. The HSP70 mRNA expression of different tissues showed that head kidney presented the fastest response to nitrite nitrogen stress, then gill, intestine and spleen. For recovering 96 h, HSP70 mRNA expression of head kidney, intestine and spleen returned to normal. The result suggested that nitrite nitrogen affected the protein metabolism, lipid metabolism and glycometabolism of A. nobilis, and also influenced the HSP70 mRNA expression of different tissues. A. nobilis showed rapid response to stress of high concentrations of water nitrite nitrogen after 6 h, so as to regulate physiological metabolism, and improve protection from stress injury and damage.关键词
鳙/亚硝酸盐氮/胁迫/血液生化指标/应激激素/HSP70Key words
Aristichthys nobilis/nitrite nitrogen/stress/blood biochemical indictors/stress hormone/HSP70引用本文复制引用
林艳,缪凌鸿,盘文静,戈贤平,任鸣春,刘波,梁化亮,赵振新..亚硝酸盐氮胁迫对鳙血液生化指标以及组织HSP70 mRNA表达水平的影响[J].水产学报,2017,41(8):1192-1202,11.基金项目
现代农业产业技术体系大宗淡水鱼专项(CARS-46) （CARS-46）
中央级公益性科研院所基本科研业务费专项(2015GBFM25) China Agriculture Research System Special Project of National Conventional Freshwater Fishery Industry (CARS-46) （2015GBFM25）
National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center of CAFS (2015GBFM25) （2015GBFM25）
