水产学报2017，Vol.41Issue(8)：1203-1212,10.DOI:10.11964/jfc.20160110236
菊黄东方鲀发育早期的脂肪酸组成变化
Changes in fatty acid composition during early developmental stage of tawny puffer (Takifugu flavidus)
摘要
Abstract
In order to understand the value of changes in fatty acid composition during early developmental stage of tawny puffer Takifugu flavidus, the fatty acid composition and content of tawny puffer in different developmental stages (unfertilized eggs, embryos 80-85 h after fertilization, newly hatched larvae 0-day-age, and before feeding larvae 3-day-age) were detected and analyzed by biochemical analysis methods. The results show that the moisture of egg and fry increased significantly with ontogenesis; the total lipid contents of fry decrease significantly with ontogenesis. 8 saturated fatty acids (SFA), 7 mono-unsaturated fatty acids (MUFA), and 12 poly-unsaturated fatty acids (PUFA) were found in the dry samples at different developmental stages. There was very similar tendency in fatty acid composition between unfertilized egg and its parent ovaries. At the embryonic period, the order of utilization rate of fatty acid was SFA (15.08%), MUFA (14.46%), n6PUFA (10.20%), and n3PUFA (0.19%), and C16:0, C16:1, C18:1n9c, and C18:2n6c were main sources of energy metabolism, and n3PUFA was preserved by priority, especially DHA, which was fully retained. After hatched, at the endogenous feeding stage, utilization rate of n3PUFA rose rapidly, followed by n3PUFA (19.60%), n6PUFA (16.98%), SFA (13.50%), and MUFA (13.01%). This was reverse to the embryo; C18:2n6c, C22:5n3(DPA), C20:5n3(EPA), C22:6n3(DHA), C18:1n9c, and C16:0 were main sources of energy metabolism, and the highest practical utilized quantity was DHA (14.44 mg/g), n3PUFA (especially DHA) was largely consumed before the larvae feeding. Therefore, larval development of tawny puffer needs to consume large amounts of n3PUFA (especially DHA and EPA). In larval breeding of tawny puffer, in order to improve the survival rate of larvae, enhancement of bait enriched with DHA and EPA (e.g., seawater rotifera, and Artemia sp. nauplii enriched with algae) was suggested.关键词
菊黄东方鲀/卵/胚胎/仔鱼/脂肪酸Key words
Takifugu flavidus/egg/embryo/larvae/fatty acid分类
生物科学引用本文复制引用
施永海,徐嘉波,刘永士,张海明,邓平平,陆根海,张宗锋..菊黄东方鲀发育早期的脂肪酸组成变化[J].水产学报,2017,41(8):1203-1212,10.基金项目
公益性行业(农业)科研专项(201203065) （农业）
上海市科学技术委员会项目(13DZ2251800) Special Fund for Agro-scientific Research in the Public Interest of China (201203065) （13DZ2251800）
Sciences and Technology Commission of Shanghai (13DZ2251800) （13DZ2251800）
