水产学报2017，Vol.41Issue(8)：1213-1222,10.DOI:10.11964/jfc.20161210639
中华绒螯蟹的血细胞组成、分类及免疫学功能
The composition and classification of hemocytes from Chinese mitten crab (Eriocheir sinensis) and primary research on immunological functions
摘要
Abstract
To study the classification, compositon and the role of different types of hemocytes in immune response in Eriochior sinensis, we researched the composition and classification of hemocytes from E. sinensis using cytochemical assays, cytoenzymatic assays and morphological observation in this investigation. Meanwhile, total hemocyte counts (THC) and different hemocyte counts (DHC) were performed. Our results showed that hemocytes from E. sinensis were divided into four types: granulocytes (G), intermediate granulocytes (IG), semigranulocyte (SG) and hyalinocytes (H). SG got the highest percentage about 33.54% while IG got the lowest only about 15.31%. Only granulocytes showed positivity to acid phosphatase, alkaline phospatase, β-glucuronidase and phenoloxidase and all types of hemocytes contain polysaccharide but no lipid. In addition, the THC increased obviously at 3 h after the injection of Aeromonas hydrophilia and reached a peak at 6 h, which was significantly higher than blank and saline groups. The numbers of granulocytes declined dramatically and hyalinocytes increased at the same time during the infection of bacteria, and this phenomenon recovered to their normal level 6 h later. Therefore, we concluded that hyalinocytes perform phagocytosis with large numbers of proliferation while granulocytes perform release of the immune-related enzymes with cell lysis during the response in non-specific immunity in E. sinensis. In addition, there might be transformations between various types of hemocytes and intermediate granulocytes were the interim types during the transformation. Hemocyte classification in crustacean had been studied by several researchers but there was no final conclusion in this field. The method of three categories was adopted by most researchers. However, the author found that there was one intermidiate type of hemocytes and it would be helpful to the research of hemocytopoiesis and immune function of different types of hemocytes.关键词
中华绒螯蟹/血细胞/细胞化学分析/细胞酶学分析/THC/DHC/嗜水气单胞菌/免疫功能Key words
Eriochior sinensis/hemocyte/cytochemical assay/cytoenzymatic assay/THC/DHC/Aerononas hydrophila/immunological function分类
农业科技引用本文复制引用
洪宇航,杨筱珍,成永旭,徐敏杰,黄根勇,杨志刚,黄毅..中华绒螯蟹的血细胞组成、分类及免疫学功能[J].水产学报,2017,41(8):1213-1222,10.基金项目
国家自然科学基金(31272677,31472287) （31272677,31472287）
水产动物遗传育种中心上海协同创新中心(ZFl206) （ZFl206）
上海市科委项目(16DZ2281200) National Natural Science Foundation of China (31272677, 31472287) （16DZ2281200）
Genetics and Breeding of Aquatic Animals Center, Shanghai Collaborative Innovation Center (ZF1206) （ZF1206）
Shanghai Committee of Science and Techonology Project (16DZ2281200) （16DZ2281200）
