水产学报2017，Vol.41Issue(8)：1319-1327,9.DOI:10.11964/jfc.20161210657
细胞骨架蛋白actin参与石斑鱼虹彩病毒SGIV释放
Actin filament involved in the release of SGIV from host cells
摘要
Abstract
The cytoskeleton such as microfilaments and microtubules play important roles in the mechanism of viral infection, replication release and virus-host cell interactions. In this study, the Singapore grouper iridovirus (SGIV) particles were purified and the viral envelopes were separated from the viral particle by detergent dissolution. The envelope proteins were identified by 1-DE-MALDI TOF and LC-MALDI TOF workflows. Except for viral proteins, 7 host proteins were found to exist in the envelope component including actin. Thus, these host proteins were co-purified with the viral particles. Actin was proved to exist with the envelope by Western-blot. Further, it was observed that the actin proteins existed on the surface of the virus particles by immnoelectron microscopy, suggesting that host original actin is packed to viral particle when SGIV is matured and finally released from the host cell. Actin may be assembled in the viral surface when virus is budding or it is specifically adhered to the viral envelope. This makes actin co-purified with the envelope proteins. Further studies indicated that the virus-infected cell turn round and the viral particles were co-located with the microfilament on the cell surface. This suggested actin was involved in viral release. When the SGIV was released from the cell, the virus obtained the cell membrane as its envelope, which was observed by electron microscopy. It was speculated that actin was specifically packed to the surface of the viral particle when SGIV was released. Together, actin played important roles when the viruses were released from the host cells.关键词
新加坡石斑鱼虹彩病毒/囊膜蛋白/actin/病毒释放Key words
Singapore grouper iridovirus/envelope protein/actin/viral release分类
农业科技引用本文复制引用
王著希,周胜,黄友华,王劭雯,黄晓红,周永灿,秦启伟..细胞骨架蛋白actin参与石斑鱼虹彩病毒SGIV释放[J].水产学报,2017,41(8):1319-1327,9.基金项目
国家自然科学基金青年基金(31502207) （31502207）
国家自然科学基金重点基金(31330082) （31330082）
广东省海洋渔业科技与产业发展专项(A201501C01) National Natural Science Foundation of China Young Scholar (31502207) （A201501C01）
Key Project of National Natural Science Foundation of China (31330082) （31330082）
Project for Guangdong Marine Fishery Science and Technology and Industry (A201501C01) （A201501C01）
