山东科学2017，Vol.30Issue(4)：1-5,5.DOI:10.3976/j.issn.1002-4026.2017.04.001
参贝消瘿颗粒的HPLC特征图谱
HPLC specific chromatogram of Shenbei Xiaoying Granules
摘要
Abstract
The HPLC specific chromatogram of Shenbei Xiaoying Granules was established.Specific chromatogram was carried out on a ODS-2 Hypersil C18 column (250mm × 4.6mm, 5μm), with gradient elution consisting of acetonitrile as mobile phase A and 0.1% phosphoric acid solution as mobile phase B.Column temperature was 30 ℃, flow rate was 1.0 mL·min-1,detection wavelength was 225 nm, and sample volume was 5 μL.The established HPLC specific chromatogram of Shenbei Xiaoying granules has good precision, repeatability and stability.The similarity of characteristic maps of 9 batches of Shenbei Xiaoying Granules was all greater than 0.95.Five common specific chromatogram peaks were determined, and two peaks were identified as paeoniflorin and rosemary acid.At the same time, the source of each characteristic peak in HPLC specific chromatogram was determined by comparing and analyzing the HPLC specific chromatogram of single medicinal herb.This method is accurate, reliable and specific, which can be used for the quality control of Shenbei Xiaoying Granules.关键词
参贝消瘿颗粒/HPLC/特征图谱Key words
Shenbei Xiaoying Granules/HPLC/specific chromatograms分类
医药卫生引用本文复制引用
张磊,马海春,苏酩,刘善新,王杰,靳光乾..参贝消瘿颗粒的HPLC特征图谱[J].山东科学,2017,30(4):1-5,5.基金项目
山东省科技发展计划(2014GGH218026) （2014GGH218026）
济南市科技计划(201219008) （201219008）
济南市高校院所计划(201401255) （201401255）
