山东科学2017，Vol.30Issue(4)：13-18,6.DOI:10.3976/j.issn.1002-4026.2017.04.003
山楂叶提取物中牡荆素、金丝桃苷、异槲皮苷对照品的制备
Preparation of reference substances of vitexin, hyperoside and isoquercitrin from Crataegi Folium extract
摘要
Abstract
Three low content flavonoids, such as vitexin, hyperoside and isoquercitrin from Crataegi Folium were rapidly separated and purified by high-speed counter-current chromatography (HSCCC) combined with pre-HPLC.The total flavonoids from Crataegi Folium were firstly separated by HSCCC, with a solvent system of chloroform/methanol/water/ n-butanol (4∶3∶2∶1.5, V/V), flow-rate of 5.0 mL/min, rotation speed of 850 r/min, detection wavelength of 254 nm.A mixed component was separated from crude flavonoids, containing vitexin, hyperoside, and isoquercitrin.Then, the mixture was separated by pre-HPLC and vitexin, hyperoside, and isoquercitrin were obtained, with the purity over 98%, respectively, as determined by HPLC.Experimental results show that it is an efficient method for separation of low content flavonoids with high purities from Crataegi Folium by HSCCC combined with pre-HPLC.关键词
山楂叶/牡荆素/金丝桃苷/异槲皮苷/高速逆流色谱/半制备液相色谱Key words
Crataegi Folium/vitexin/hyperoside/isoquercitrin/HSCCC/pre-HPLC分类
医药卫生引用本文复制引用
文蕾,林云良,高红梅,李峰,姜姣姣,宋祥云,付瑞明,王岱杰..山楂叶提取物中牡荆素、金丝桃苷、异槲皮苷对照品的制备[J].山东科学,2017,30(4):13-18,6.基金项目
国家自然科学基金((81473298, 21506119) （(81473298, 21506119）
山东省科技发展计划(2014GZX219003) （2014GZX219003）
山东省三院联合基金(ZR2016YL006) （ZR2016YL006）
